(E/Z)-TG003 NEW
| Price | $41 | $58 | $81 |
| Package | 1mg | 2mg | 5mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2024-11-14 |
Product Details
| Product Name: (E/Z)-TG003 | CAS No.: 300801-52-9 |
| Purity: 99.04% | Supply Ability: 10g |
| Release date: 2024/11/14 |
Product Introduction
Bioactivity
| 名稱 | (E/Z)-TG003 |
| 描述 | (E/Z)-TG003 is a potent and ATP-competitive inhibitor of Cdc2-like kinase (Clk). |
| 細胞實驗 | 2 × 105 HeLa cells or 1.5 × 105 COS-7 cells resuspended in 2 mL of medium are plated on 6-well dishes, and 2 μL of 10 mM TG003 dissolved in Me2SO (final concentration at 10 μM), or 2 μL of Me2SO, is added to some wells. Cells are trypsinized, and the density is counted every 24 h for 3 days. Cells are then fixed with 1 mL of ice-cold 70% ethanol, washed with PBS, incubated in 1 ml of PBS containing 1 μg/mL DNase-free RNase A and 50 μg/mL propidium iodide for 20 min at 37 °C, and proceeded to cell cycle analysis by FACSCalibur.(Only for Reference) |
| 激酶實驗 | In Vitro Kinase Assay : Kinase activity of Clks and SRPKs is assayed in a reaction mixture, containing 200 mM Tris-HCl (pH 7.5), 12.5 mM MgCl2, 8 mM dithiothreitol, 4 mM EGTA, 1–20 μM ATP, 1 μCi of [γ-32P]ATP, 1 μg of synthetic peptide of SF2/ASF RS domain (NH2-RSPSYGRSRSRSRSRSRSRSRSNSRSRSY-OH), and 0.1–1 μg of purified kinases in a final volume of 40 μL. cAMP-dependent protein kinase activity is assayed in a reaction mixture containing 80 mM Tris-HCl (pH 7.5), 12.5 mM MgCl2, 8 mM dithiothreitol, 4 mM EGTA, 10 μM ATP, 1 μCi of [γ-32P]ATP, 5 μg of histone H1, and 1 μg of catalytic subunit of rat cAMP-dependent protein kinase purified. Protein kinase C activity is assayed in a reaction mixture containing 200 mM Tris-HCl (pH 7.5), 12.5 mM MgCl2, 1 mM CaCl2, 80 μg/mL phosphatidylserine, 8 μg/mL diolein, 10 μM ATP, 1 μCi of [γ-32P]ATP, 5 μg of histone H1, and 2 μL of partially purified rat protein kinase C. The final concentration of Me2SO is adjusted to 1% regardless of inhibitor concentration. The reaction mixture is incubated at 30 or 25 °C for mammalian or Xenopus recombinant proteins, respectively, for 10 min, and a half-portion is spotted on P81 phosphocellulose membrane. The kinase assay conditions, including the incubation period and concentration of kinases and substrates, are optimized to maintain the linearity during incubation. The membrane is washed with 5% phosphoric acid solution (SF2/ASF RS domain) or 5% trichloroacetic solution (histone H1) at least over 15 min. The radioactivity is measured using a liquid scintillation counter. The net radioactivity is deduced by subtracting the background count from the reaction mixture without kinase, and the data are expressed as the percentage to the control sample containing the solvent. |
| 體外活性 | At a concentration of 10 μM, TG003 is capable of ameliorating embryonic defects in Xenopus laevis induced by excessive Clk activity within the organism. |
| 體內(nèi)活性 | TG003 inhibits the splicing of heteronuclear RNA of IL-1β, thereby blocking the production of IL-1β RNA in platelets. During the differentiation process of 3T3-L1 adipocytes, TG003 also obstructs the alternative splicing of PKCβII and the expression of PPARγ1 and PPARγ2. Furthermore, it inhibits the in vitro SF2/ASF-dependent splicing of human β-globin by suppressing the phosphorylation mediated by Clk1/Sty. TG003 inhibits the kinase activity of Clk1/Sty in mammalian cells without exhibiting toxic effects on the growth of HeLa and COS-7 cells at a concentration of 10 μM. |
| 存儲條件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | DMSO : 6 mg/mL (24.06 mM), Heating is recommended. Ethanol : < 1 mg/mL (insoluble or slightly soluble) |
| 關(guān)鍵字 | (E/Z) TG003 | Cyclin dependent kinase | (E/Z)-TG-003 | site | tumor | Wilm's | TG-003 | splice | inhibit | mutation | Inhibitor | TG 003 | CDK | (E/Z)-TG003 | cancer | (E/Z)TG003 | (E/Z)-TG 003 | breast |
| 相關(guān)產(chǎn)品 | Ribociclib | (S)-(+)-Ibuprofen | Navitoclax | Palbociclib monohydrochloride | Venetoclax | CASIN | Palbociclib | GW 441756 | Sodium Oxamate | Abemaciclib | Dinaciclib | Abemaciclib methanesulfonate |
| 相關(guān)庫 | 細胞凋亡化合物庫 | 經(jīng)典已知活性庫 | 激酶抑制劑庫 | 抗乳腺癌化合物庫 | 抑制劑庫 | NO PAINS 化合物庫 | PPI抑制劑庫 | 抗衰老化合物庫 | 已知活性化合物庫 | 抗癌化合物庫 |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
You may like
Recommended supplier
| Product name | Price | Suppliers | Update time | |
|---|---|---|---|---|
| $73.00/1mg |
VIP3Y
|
TargetMol Chemicals Inc.
|
2024-11-14 | |
| $1230.00/200mg |
VIP1Y
|
TargetMol Chemicals Inc.
|
2024-10-28 | |
| $1.00/1g |
VIP5Y
|
Career Henan Chemica Co
|
2020-11-12 |
United States- Since: 2011-01-07
- Address: 36?Washington?Street, Wellesley?Hills
INQUIRY