Coelenterazine NEW
| Price | $97 | $148 | $319 |
| Package | 1mg | 2mg | 5mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2024-11-04 |
Product Details
| Product Name: Coelenterazine | CAS No.: 55779-48-1 |
| Purity: 99.02% | Supply Ability: 10g |
| Release date: 2024/11/04 |
Product Introduction
Bioactivity
| 名稱 | Coelenterazine |
| 描述 | Coelenterazine is a superoxide anion-sensitive chemiluminescent probe that can be used to detect the chemiluminescence of peroxynitrite and can serve as a luminescent enzyme substrate for apoaequorin and Renilla luciferase. |
| 細(xì)胞實驗 | Cells are plated at a density of 5×10^4?cells per well into 24-well plates and grown to 80-100% confluency. Just before imaging, media are changed to a colorless solution containing (in mM): 2.7 KCl, 139 NaCl, 8.1 Na2HPO4, 0.7 H2O, 1.5 KH2PO4, 1.8 CaCl2, 1 MgCl2?and 5.5 d-glucose. Cells are preincubated for 15 min in the absence or presence of Pgp modulator, after which Coelenterazine (final concentration of 470 nM) is added directly to the cells. They are for reference only. |
| 動物實驗 | Mice are anesthetized with metofane or isoflurane before tail vein injection of Coelenterazine (4 μg/g) formulated from an ethanol stock diluted in sodium phosphate buffer (50 mM). Bioluminescence imaging is performed on the in vivo imaging system at 2, 6, 8, and 11 min after injection. Anesthesia is maintained during imaging by nose cone delivery of 2.5% isoflurane. After imaging, animals are killed by cervical dislocation; tumors are then harvested and weighed. They are for reference only. |
| 體外活性 | When monitoring various biological processes, coelenterazine (CTZ) can be catalyzed by luciferases, such as the sea pansy Renilla reniformis (Rluc) and the marine copepod Gaussia princeps (Gluc), which results in emission of blue light. In Gli36-Gluc- or Gli36-Rluc-expressing Gli36 cells, coelenterazine (8 礸/ml) shows a bioluminescence signal. [1] In RIN-5F, 4T1, HCT 116, HEK 293, 293FT, HeLa and INS-1 cells, coelenterazine can be used to assay superoxide anion concentrations. [2] |
| 體內(nèi)活性 | METHODS: Gli36-Gluc and Gli36-Rluc cells were injected into the leg muscles of nude mice. Mice were injected intravenously with 100 μg (4 mg/kg body weight) of coelenterazine and immediately imaged using a cooled charge-coupled device (CCD) camera. The mice were injected with 500 μg (20 mg/kg) of water-soluble coelenterazine and imaged using a CCD camera. RESULTS Water-soluble coelenterazine produced 10-fold higher photon counts for Gluc and 4-fold higher photon counts for Rluc, indicating that s-coelenterazine is more sensitive for detecting coelenteric acid luciferase in vivo. [1] |
| 存儲條件 | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | Ethanol : 1 mg/mL (2.36 mM), DMSO inactivates the activity of Coelenterazine. H2O : < 1 mg/mL (insoluble or slightly soluble) |
| 關(guān)鍵字 | inhibit | Inhibitor | Coelenterazine |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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United States- Since: 2011-01-07
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