Aprotinin NEW
| Price | $39 | $54 | $117 |
| Package | 5mg | 10mg | 25mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2024-11-15 |
Product Details
| Product Name: Aprotinin | CAS No.: 9087-70-1 |
| Supply Ability: 10g | Release date: 2024/11/15 |
Product Introduction
Bioactivity
| 名稱 | Aprotinin |
| 描述 | Aprotinin (Traskolan) is a broad-spectrum serine protease (BPTI) inhibitor that inhibits the activity of a number of different esterases and proteases. Aprotinin is an antifibrinolytic agent used to minimize hemorrhage during complex surgical procedures. |
| 細(xì)胞實(shí)驗(yàn) | Mouse G8-1 myoblasts are plated DMEM + 20% FBS (maintenance medium), in which they remain undifferentiated. When cells reach approximately 40-50% confluence, different protease inhibitors are added to the culture media and cells are incubated overnight. Cells are then switched to differentiation-promoting media (DMEM + 10% horse serum ± protease inhibitor) and incubated for 7 days. (Only for Reference) |
| 激酶實(shí)驗(yàn) | Substrates and kinases are diluted in 50?mM Tris/HCl (pH?7.5), 0.1% 2-mercaptoethanol, 0.1?mM EGTA and 10?mM magnesium acetate. Reactions are initiated with [γ-32P]ATP (2500 c.p.m./pmol) to a final concentration of 0.1?mM and terminated after 15?min at 30°C by the addition of SDS and EDTA (pH?7.0) to final concentrations of 1.0% (w/v) and 20?mM respectively. After heating for 5?min at 100°C and separation by SDS/PAGE, the phosphorylated proteins are detected by autoradiography. |
| 體外活性 | METHODS: Hepatocellular carcinoma cell lines Hep3B and HCCLM3 were treated with Cepharanthine (1-100 μM) for 48 h and cell viability was measured by CCK-8 assay. RESULTS: Cepharanthine treatment for 48 h significantly inhibited the viability of Hep3B and HCCLM3 cell lines in a dose-dependent manner at a value of approximately 20 μM. [1] METHODS: The human hepatocellular carcinoma cell line HuH-7 was treated with Cepharanthine (20 μM) for 7.5-240 min, and the expression levels of target proteins were detected by Western Blot. RESULTS: Cepharanthine induced time-dependent phosphorylation of JNK and p38 MAPK. c-Jun (Ser 73) and ATF-2, downstream targets of JNK and p38, were significantly activated by Cepharanthine. Cepharanthine caused significant activation of ERK1/2, while Akt was down-regulated by Cepharanthine. [2] |
| 體內(nèi)活性 | METHODS: To detect anti-tumor activity in vivo, Cepharanthine (10-20 mg/kg) was injected intraperitoneally into nude mice bearing Hep3B xenografts every two days for 24 days. RESULTS: Cepharanthine treatment significantly prolonged the survival time of nude mice with transplanted tumors. Cepharanthine treatment significantly inhibited the growth of transplanted tumors in vivo. [1] |
| 存儲(chǔ)條件 | keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | H2O : 100 mg/mL (15.36 mM), Sonication is recommended. |
| 關(guān)鍵字 | Ser/Thr Protease | Inhibitor | Aprotinin | Serine proteases | Serine endopeptidases | inhibit | Threonine proteases | Influenza Virus |
| 相關(guān)產(chǎn)品 | Curcumin | Crystal Violet |
| 相關(guān)庫(kù) | HIF-1化合物庫(kù) | 蛋白酶抑制劑庫(kù) | 多肽分子庫(kù) | NO PAINS 化合物庫(kù) | 抗感染化合物庫(kù) |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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United States- Since: 2011-01-07
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