Amphotericin B NEW
| Price | $31 | $44 | $76 |
| Package | 50mg | 100mg | 500mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2024-11-19 |
Product Details
| Product Name: Amphotericin B | CAS No.: 1397-89-3 |
| Purity: ≥98% | Supply Ability: 10g |
| Release date: 2024/11/19 |
Product Introduction
Bioactivity
| Name | Amphotericin B |
| Description | Amphotericin B (NSC-527017) is a polyene antifungal agent with broad-spectrum activity against many fungal species. Amphotericin B irreversibly binds to ergosterol and disrupts the integrity of cell membranes, resulting in antifungal activity. |
| Cell Research | Amphotericin B is dissolved in DMSO. The kinetics of cell death induced by AmB against Leishmania promastigotes is followed by using fluorometry with the DNA-binding compound ethidium bromide (EB). Fluorescence measurements are performed on a SPEX Fluorolog II spectrophotometer at 365-580 nm excitation-emission wavelengths. Promastigotes at a final concentration of 25×106 cells/mL are incubated for 5 min with gentle stirring in the fluorescence cuvette with 2 mL of different buffered solutions but always containing 10 mM glucose and EB (50 mM). After signal stabilization is achieved, AmB is added and dissolved in dimethylsulfoxide. Maximal EB incorporation is always obtained by adding digitonin (50 mg/mL). All solutions used are buffered with 75 mM TRIS (pH 4 7.6) and contain 150 mM NaCl (BNa+), 150 mM KCl (BK+), 150 mM choline chloride, and 100 mM sucrose, 100 mM NaCl. The osmolarity of all solutions is always adjusted to 390±5 mOsm using an advanced instrument SW2 osmometer. |
| Kinase Assay | THP-1 and HEK293 cells are transiently transfected using DEAE-dextran and Polyfect reagent, respectively. Plasmids transfected contain genes coding for the NF-κB-dependent pELAM-luc luciferase reporter, TLR2, TLR4, CD14, and MD2. Cells (5×105 THP-1 or 1×105 HEK293) are added to 12-well plates, washed after 18 h, and stimulated for 5 h. Cells are then lysed with reporter lysis buffer as directed, and lysates are analyzed for luminescence using Promega luciferase substrate and a Monolight 3010 luminometer. |
| In vitro | METHODS: Human normal colon epithelial cells CCD 841 CoTr and human colorectal cancer cells HT-29 were treated with Amphotericin B (0.05-25 μg/mL) for 24 h. Cell viability was measured by Neutral Red method. RESULTS: Higher concentrations of Amphotericin B were toxic to CCD 841 CoTr and HT-29 cells, with IC50 values of 8.7 μg/mL and 21.2 μg/mL, respectively. [1] METHODS: Candida albicans were treated with Amphotericin B (100 μM) for 4-16 min and imaged using the FLIM technique. RESULTS: Amphotericin B preferentially binds to the cell wall and does not efficiently cross the barrier covering the cell membrane.Amphotericin B can more readily pass through the cell wall barrier of young cells during the emergence stage. [2] |
| In vivo | METHODS: To assay antifungal activity in vivo, Amphotericin B (0.25-4 mg/kg) was administered as a single intraperitoneal injection to C. albicans K-1-infected ICR/Swiss mice. RESULTS: Only the highest single dose of Amphotericin B treatment significantly reduced the number of colonies compared to the number of colonies at the start of treatment. [3] |
| Storage | store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | H2O : < 1 mg/mL (insoluble or slightly soluble) 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 5 mg/mL (5.41 mM), Suspension. Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. DMSO : 50 mg/mL (54.11 mM) Ethanol : < 1 mg/mL (insoluble or slightly soluble) |
| Keywords | NSC-527017 | NSC527017 | Inhibitor | inhibit | Fungal | Antibiotic | Amphotericin B |
| Inhibitors Related | Neomycin sulfate | Dehydroacetic acid sodium | Potassium gluconate | Ampicillin sodium | Doxycycline (hyclate) | Kanamycin sulfate | G-418 disulfate | Sulfamethoxazole sodium | Metronidazole | Doxycycline | Geraniol | EDTA copper(II) disodium salt |
| Related Compound Libraries | FDA-Approved & Pharmacopeia Drug Library | Bioactive Compound Library | Anti-Fungal Compound Library | Natural Product Library | Drug Repurposing Compound Library | Microbial Natural Product Library | Antiparasitic Natural Product Library | FDA-Approved Drug Library | Natural Product Library for HTS | Anti-infective Natural Product Library | Immunology/Inflammation Compound Library | Bioactive Compounds Library Max |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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