Description of Acid Blue 90 from Wuhan Fortuna Chemical Co.,Ltd

Specifications(COA) of Chemical raw material Acid Blue 90 Powder CAS 6104-58-1 for Dye

Introduction of Acid Blue 90

Acid Blue 90, also known as Coomassie Brilliant Blue R-250, is a synthetic dye commonly used in biochemical laboratories, particularly for staining proteins in various types of electrophoresis gels, such as SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). Here are some key details about Acid Blue 90:

Chemical Information:

Chemical Formula: C45H44N3NaO7S2

Molecular Weight: 825.97 g/mol

CAS Number: 6104-58-1

Applications:

Protein Staining: Acid Blue 90 is widely used for staining proteins in polyacrylamide gels. The dye binds to proteins, allowing them to be visualized as distinct bands. This is particularly useful for analyzing protein purity, molecular weight, and concentration.

Bradford Protein Assay: Acid Blue 90 is a key component of the Bradford protein assay, a colorimetric assay used to measure the concentration of proteins in a solution. When the dye binds to proteins, it undergoes a shift in absorbance maximum, which can be measured spectrophotometrically.

Histology and Cytology: In addition to its use in protein analysis, Acid Blue 90 can also be used for staining tissues and cells in histological and cytological studies.

Properties:

Color: Acid Blue 90 is a dark blue dye.

Solubility: It is soluble in water and ethanol.

Binding: The dye binds to proteins primarily through ionic interactions and hydrophobic interactions, which makes it effective for staining purposes.

Advantages:

Sensitivity: Acid Blue 90 is highly sensitive and can detect small amounts of protein.

Ease of Use: The staining process is relatively straightforward and does not require complex procedures.

Reproducibility: The results obtained using Acid Blue 90 are highly reproducible, making it a reliable choice for protein analysis.

Procedure for Protein Staining:

Preparation: Prepare the staining solution by dissolving Acid Blue 90 in an appropriate solvent, often a mixture of methanol, acetic acid, and water.

Staining: After running the electrophoresis gel, immerse the gel in the staining solution and incubate for a specified period, usually a few hours.

Destaining: Remove excess dye by washing the gel with a destaining solution, typically containing methanol and acetic acid, until the background is clear and the protein bands are distinctly visible.

Safety:

Handling: As with all chemical reagents, handle Acid Blue 90 with care, using appropriate personal protective equipment (PPE) such as gloves and safety goggles.

Disposal: Dispose of the dye and any solutions containing it according to local regulations and guidelines for hazardous waste.

In summary, Acid Blue 90 (Coomassie Brilliant Blue R-250) is a versatile and widely used dye in biochemical research for staining proteins in electrophoresis gels and for protein quantification assays. Its high sensitivity, ease of use, and reproducibility make it an essential tool in protein analysis.

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