Description of 4-Nitrophenyl β-D-Cellobioside from Wuhan Fortuna Chemical Co.,Ltd

Specifications(COA) of High Quality 98.2% 4-Nitrophenyl β-D-Cellobioside CAS NO 3482-57-3 Biochemical in Stock

Introduction of 4-Nitrophenyl β-D-Cellobioside.

4-Nitrophenyl β-D-Cellobioside is a synthetic substrate commonly used in biochemical assays to study the activity of cellulase enzymes. It is a derivative of cellobiose, which is a disaccharide composed of two glucose molecules linked by a β(1→4) glycosidic bond. In this compound, the reducing end of the cellobiose is modified with a 4-nitrophenyl group.

When cellulase enzymes act on 4-Nitrophenyl β-D-Cellobioside, they cleave the glycosidic bond, releasing 4-nitrophenol. The release of 4-nitrophenol can be easily monitored because it has a distinct yellow color and absorbs light at a wavelength of 405 nm. This property makes 4-Nitrophenyl β-D-Cellobioside a useful tool for measuring cellulase activity in various research and industrial applications.

In summary, 4-Nitrophenyl β-D-Cellobioside is a valuable substrate for studying cellulase enzymes, providing a convenient and quantifiable way to assess enzyme activity through the release of 4-nitrophenol.

Application of 4-Nitrophenyl β-D-Cellobioside.

4-Nitrophenyl β-D-Cellobioside is primarily used in biochemical and enzymatic assays to study the activity of cellulase enzymes. Here are some specific applications:

Enzyme Activity Assays: It is used to measure the activity of cellulase enzymes. When cellulases cleave the glycosidic bond in 4-Nitrophenyl β-D-Cellobioside, they release 4-nitrophenol, which can be quantitatively measured by its absorbance at 405 nm. This allows researchers to determine the rate of enzyme activity.

Enzyme Kinetics: Researchers use this substrate to study the kinetics of cellulase enzymes, including parameters like Km (Michaelis constant) and Vmax (maximum velocity). This helps in understanding the efficiency and mechanism of the enzyme.

Screening for Enzyme Inhibitors: It can be used in high-throughput screening assays to identify potential inhibitors of cellulase enzymes. By observing changes in the release of 4-nitrophenol, researchers can determine the effectiveness of various compounds in inhibiting cellulase activity.

Characterization of Mutant Enzymes: In genetic and protein engineering studies, 4-Nitrophenyl β-D-Cellobioside can be used to characterize the activity of mutant cellulase enzymes. This helps in understanding how specific mutations affect enzyme function.

Industrial Applications: In industries such as biofuel production, where cellulases are used to break down cellulose into fermentable sugars, 4-Nitrophenyl β-D-Cellobioside can be used to monitor and optimize enzyme performance.

Environmental Studies: It can be used to study the degradation of cellulose in various environmental samples, helping to understand the role of cellulases in natural ecosystems.

Quality Control: In the production of cellulase enzymes, 4-Nitrophenyl β-D-Cellobioside can be used for quality control to ensure that the enzyme preparations have the desired activity.

Overall, 4-Nitrophenyl β-D-Cellobioside is a versatile and valuable tool in both research and industrial settings for studying and utilizing cellulase enzymes.

Wuhan Fortuna Chemical Co.,Ltd established in 2006, is a big integrative chemical enterprise being engaged in Pharmaceutical and Intermediates, Food and Feed additives, Fine chemicals,Biochemical and Reagent, which is  the members of Hubei E-commerce Chamber.

Our company has professional persons who major in chemical R&D and sicentific management, supply fine chemical products with high quality and close service, also supply custom-tailored products according to our clients' requirement.

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Since Wuhan Fortuna Chemical Co., Ltd founded, we continuously innovate our products and improve our service, sales network. Hence, we initiate the first sale mode on net in China, which is the retail trade of small package bring along wholesale of diversified management modes. Our products are exported widely to South Korea, Vietnam, Australia, Europe and South America, highly recommended by our clients.We insist on the management creed “Market is our compass, Quality is our life, Credit is our soul”. Clients’ trust is our forward power, their satisfaction is our struggling goal.

Wuhan Fortuna Chemical Co.,Ltd is a professional manufacturer which provide 4-Nitrophenyl β-D-Cellobioside with good quality and best price, welcome to contact us to get COA/TDS/MSDS of 4-Nitrophenyl β-D-Cellobioside.