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| | 5(6)-Carboxyfluorescein Basic information |
| | 5(6)-Carboxyfluorescein Chemical Properties |
| Melting point | 275 °C | | Boiling point | 464.9°C (rough estimate) | | density | 1.3418 (rough estimate) | | refractive index | 1.6380 (estimate) | | storage temp. | Keep in dark place,Inert atmosphere,Room temperature | | solubility | DMSO (Sparingly), Methanol (Slightly, Heated) | | form | Solid | | color | Light Yellow to Dark Brown | | Water Solubility | slightly soluble | | InChIKey | CPRVUBZYHZGASQ-UHFFFAOYSA-N | | SMILES | C12(OC(=O)C3C=CC(C(=O)O)=CC1=3)C1=CC=C(O)C=C1OC1C=C(O)C=CC2=1.C12(OC(=O)C3C=C(C(=O)O)C=CC1=3)C1=CC=C(O)C=C1OC1C=C(O)C=CC2=1 | | EPA Substance Registry System | |
| | 5(6)-Carboxyfluorescein Usage And Synthesis |
| Description | 5(6)-Carboxyfluorescein is a mixture of 5-carboxy and 6-carboxy derivatives of fluorescein. It is commonly used to label biomolecules through a reaction involving the carboxyl group. Unlike its succinimidyl ester derivative CFSE , 5(6)-carboxyfluorescein is membrane impermeant. As a result, it can be used in studies of membrane permeability. 5(6)-Carboxyfluorescein displays excellent fluorescence (excitation/emission at 492/514 nm, respectively), and its excitation maximum closely matches the 488 nm spectral line of argon-ion lasers. | | Chemical Properties | Dark-Orange to Brown Solid | | Uses | As a popular probe of determination of intracellular pH, 5(6)-Carboxyfluoresceincan be used as useful reagent for the preparation of hydrolytically stable fluorescent conjugates and a useful starting material for the synthesis of other fluorescein-derives reagent | | Uses | 5(6)-FAM, A useful reagent for the preparation of hydrolytically stable fluorescent conjugates and is a useful starting material for the synthesis of other fluorescein-derives reagent. | | Uses | A popular probe of determination of intracellular pH. | | Definition | ChEBI: 5(6)-carboxyfluorescein is a commercially available mixture of 5-carboxyfluorescein and 6-carboxyfluorescein isomers. A fluorescent dye, it is commonly used as a tracer agent. It has a role as a tracer and a fluorescent dye. It contains a 5-carboxyfluorescein and a 6-carboxyfluorescein. | | Solubility in organics | 5(6)-Carboxyfluorescein is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide. The solubility of 5(6)-carboxyfluorescein in these solvents is approximately 5, 0.5, and 1 mg/ml, respectively. |
| | 5(6)-Carboxyfluorescein Preparation Products And Raw materials |
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