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ChemicalBook >> CAS DataBase List >>Coelenterazine 400a (hydrochloride)

Coelenterazine 400a (hydrochloride)

CAS No.
2320429-05-6
Chemical Name:
Coelenterazine 400a (hydrochloride)
Synonyms
Coelenterazine 400a (hydrochloride);6-phenyl-2,8-bis(phenylmethyl)-imidazo[1,2-a]pyrazin-3(7H)-one,monohydrochloride
CBNumber:
CB94666986
Molecular Formula:
C26H22ClN3O
Molecular Weight:
427.92538
MDL Number:
MOL File:
2320429-05-6.mol
Last updated:2023-04-23 13:52:06

Coelenterazine 400a (hydrochloride) Properties

solubility DMF: 0.5 mg/ml; DMSO: 0.5 mg/ml; Ethanol: slightly soluble
form A crystalline solid

Coelenterazine 400a (hydrochloride) price More Price(3)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Cayman Chemical 25738 Coelenterazine 400a (hydrochloride) ≥95% 2320429-05-6 500μg $81 2024-03-01 Buy
Cayman Chemical 25738 Coelenterazine 400a (hydrochloride) ≥95% 2320429-05-6 1mg $143 2024-03-01 Buy
Cayman Chemical 25738 Coelenterazine 400a (hydrochloride) ≥95% 2320429-05-6 5mg $632 2024-03-01 Buy
Product number Packaging Price Buy
25738 500μg $81 Buy
25738 1mg $143 Buy
25738 5mg $632 Buy

Coelenterazine 400a (hydrochloride) Chemical Properties,Uses,Production

Description

Coelenterazine 400a is a bisdeoxy derivative of coelenterazine that displays an emission maximum of 395 nm following conversion by Renilla luciferase (Rluc).1,2 It is used in bioluminescence resonance energy transfer 2 (BRET2) protocols, whereas coelenterazine h , which displays an emission maximum of 475 nm upon conversion by Rluc, is used in BRET1 protocols.3 Coelenterazine 400a is commonly paired with class 1 and 3 GFP acceptors, including GFP2 and GFP10.2,3 BRET2 assays are commonly used in evaluating protein-protein interactions, including those involved in G protein-coupled receptor signaling.1,2,4

References

1. Bertrand, L., Parent, S., Caron, M., et al. The BRET2/arrestin assay in stable recombinant cells: A platform to screen for compounds that interact with G protein-couple receptors (GPCRS) J. Recept. Signal Transduct. Res. 22(1-4),533-541(2002).
2. Galés, C., Rebois, R.V., Hogue, M., et al. Real-time monitoring of receptor and G-protein interactions in living cells Nat. Methods 2(3),177-184(2005).
3. Pfleger, K.D., and Eidne, K.A. Illuminating insights into protein-protein interactions using bioluminescence resonance energy transfer (BRET) Nat. Methods 3(3),165-174(2006).
4. Huang, Q., Acha, V., Yow, R., et al. Bioluminescence measurements in mice using a skin window J. Biomed. Opt. 12(5):054012,(2007).

Coelenterazine 400a (hydrochloride) Preparation Products And Raw materials

Raw materials

Preparation Products

Coelenterazine 400a (hydrochloride) Suppliers

Global( 6)Suppliers
Supplier Tel Email Country ProdList Advantage
Shanghai Hongye Biotechnology Co. Ltd 400-9205774 sales@glpbio.cn China 6777 58
ChemeGen(Shanghai) Biotechnology Co.,Ltd. 18818260767 sales@chemegen.com China 11218 58
Shanghai Yifei Biotechnology Co. , Ltd. 021-65675885 18964387627 customer_service@efebio.com China 11974 58
Cayman Chemical Company (800)364-9897 Caymanname@qq.com CHINA 21570 58
Coelenterazine 400a (hydrochloride) 6-phenyl-2,8-bis(phenylmethyl)-imidazo[1,2-a]pyrazin-3(7H)-one,monohydrochloride 2320429-05-6