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ChemicalBook >> CAS DataBase List >>Iodonitrotetrazolium chloride

Iodonitrotetrazolium chloride

CAS No.
146-68-9
Chemical Name:
Iodonitrotetrazolium chloride
Synonyms
INT;INT DYE;IntA.R.;INT CHLORIDE;2-(p-iodophe;I.N.T. 95% AR;IODONITROTETRAZOLIUM;IODONITROTETRAZOLIUM VIOLET;Iodonitrotetrazolium purple;IODONITRO TETRAZOLIUM (INT)
CBNumber:
CB7232772
Molecular Formula:
C19H13ClIN5O2
Molecular Weight:
505.7
MDL Number:
MFCD00011961
MOL File:
146-68-9.mol
MSDS File:
SDS
Last updated:2024-10-31 18:15:48

Iodonitrotetrazolium chloride Properties

Melting point 240 °C (dec.)(lit.)
storage temp. 2-8°C
solubility methanol: water (1:1): 50 mg/mL hot, very faintly turbid, very deep yellow
form Powder, Crystals and/or Chunks
color White to off-white
Water Solubility soluble
Sensitive Light Sensitive
λmax 248nm
BRN 4093224
Stability Stable. Incompatible with strong oxidizing agents.
Biological Applications Bacterial vaginosis diagnosis assay; dehydrogenase enzyme assay; diagnostic assay; food and beverage analytes assays; microbial growth assay; detecting bacteria,yeast,fungi,gamma-hydroxybutyric acid (GHB),microbial growth; measuring niacin,bacterial respiratory activity,superoxide dismutase;treating cancer
InChI InChI=1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1
InChIKey JORABGDXCIBAFL-UHFFFAOYSA-M
SMILES [N+]1(=NC(C2C=CC=CC=2)=NN1C1C=CC(I)=CC=1)C1=CC=C([N+]([O-])=O)C=C1.[Cl-]
CAS DataBase Reference 146-68-9(CAS DataBase Reference)
FDA UNII XY3JA5594E
EPA Substance Registry System 2H-Tetrazolium, 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-, chloride (146-68-9)

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictogramsGHS hazard pictograms
GHS02,GHS07
Signal word  Danger
Hazard statements  H228-H335
Precautionary statements  P210-P240-P241-P261-P271-P280
Hazard Codes  Xn,F,Xi
Risk Statements  20/21/22-68/20/21/22-36/37/38-11
Safety Statements  36/37-36/37/39-26-22-16
RIDADR  2811
WGK Germany  3
F  3-8-10
TSCA  Yes
HazardClass  6.1
PackingGroup  III
HS Code  29339900
NFPA 704
1
2 1

Iodonitrotetrazolium chloride price More Price(39)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 58030 Iodonitrotetrazolium chloride BioReagent, ≥97.0% (calc. on dry substance, NT) 146-68-9 1g $218 2024-03-01 Buy
Sigma-Aldrich 58030 Iodonitrotetrazolium chloride BioReagent, ≥97.0% (calc. on dry substance, NT) 146-68-9 5G $763 2024-03-01 Buy
Sigma-Aldrich 58030 Iodonitrotetrazolium chloride BioReagent, ≥97.0% (calc. on dry substance, NT) 146-68-9 25g $2560 2024-03-01 Buy
TCI Chemical I0067 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium Chloride >98.0%(HPLC)(T) 146-68-9 1g $60 2024-03-01 Buy
TCI Chemical I0067 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium Chloride >98.0%(HPLC)(T) 146-68-9 5g $189 2024-03-01 Buy
Product number Packaging Price Buy
58030 1g $218 Buy
58030 5G $763 Buy
58030 25g $2560 Buy
I0067 1g $60 Buy
I0067 5g $189 Buy

Iodonitrotetrazolium chloride Chemical Properties,Uses,Production

Description

Iodonitrotetrazolium (INT) (chloride) is a monotetrazolium salt used as an indicator dye. It is reduced to an insoluble formazan that is used as a vital dye or indicator of cellular redox activity. Reduction commonly results from the activity of dehydrogenases, although non-enzymatic electron transfer reactions can occur in the presence of an intermediate electron acceptor. INT is commonly used to measure the respiratory activity of microorganisms in a variety of contexts.

Chemical Properties

slightly yellow powder

Uses

Iodonitrotetrazolium chloride (INT) on reduction gets converted to iodonitrotetrazolium formazan, a water insoluble violet colored dye or indicator. Hence INT can be used for the colorimetric assay of various dehydrogenases.

Uses

Iodonitrotetrazolium chloride has been used for the staining of cells. It has been used as a coupling agent in the enzymatic assay of D-arabinitol.

Uses

Electron acceptor for the colorimetric assay of various dehydrogenases.

Definition

ChEBI: An organic chloride salt having iodonitrotetrazolium as the counterion.

Safety

Iodonitrotetrazolium chloride could cause skin irritation, serious eye irritation, and may cause respiratory irritation.

Properties and Applications

INT (Iodonitrotetrazolium chloride), similar to tetrazolium chloride on reduction, produces a red formazan dye that can be used for quantitative redox assays. It is also toxic to prokaryotes.INT is an artificial electron acceptor that can be utilized in a colorimetric assay to determine protein concentration in a solution. It can be reduced by succinate dehydrogenase to furazan, the formation of which can be measured by absorbance at 490 nm. The activity of succinate dehydrogenase is readily observed by the naked eye as the solution turns from colorless to rusty red.

Purification Methods

Recrystallise the chloride from H2O, aqueous EtOH or EtOH/Et2O. Alternatively, dissolve it in the minimum volume of EtOH and add Et2O; or dissolve it in hot H2O (charcoal), filter and precipitate it by adding conc HCl. Filter the solid off and dry it at 100o. Its solubility in H2O at 25o is 0.5%, and in hot MeOH/H2O (1:1) it is 5%. [Fox & Atkinson J Am Chem Soc 72 3629 1950, Beilstein 26 III/IV 1776.]

Iodonitrotetrazolium chloride Preparation Products And Raw materials

Raw materials

Preparation Products

Global( 312)Suppliers
Supplier Tel Email Country ProdList Advantage
Suzhou BEC Biological Technology Co., Ltd.
+undefined13771885869 all@bek.com.cn China 62 58
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BOC Sciences 		+1-631-485-4226 inquiry@bocsci.com United States 19553 58
Chongqing Chemdad Co., Ltd 		+86-023-6139-8061 +86-86-13650506873 sales@chemdad.com China 39894 58

View Lastest Price from Iodonitrotetrazolium chloride manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
INT pictures 2024-10-31 INT
146-68-9
 US $41.00 / mg 99.43% 10g TargetMol Chemicals Inc.
2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride pictures 2019-07-06 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
146-68-9
 US $100.00 / KG 1KG 99% 20kg Career Henan Chemical Co
2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride pictures 2019-07-06 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
146-68-9
 US $100.00 / KG 1KG 99% 20kg Career Henan Chemical Co
  • INT pictures
  • INT
    146-68-9
  • US $41.00 / mg
  • 99.43%
  • TargetMol Chemicals Inc.
3-(4-iodophenyl)-2-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-iuM chloride IodonitrotetrazoliuM chloride (INT) IodonitrotrtrazoliuM chloride Iodonitrotetrazolium chloride 95% Iodonitrotetrazolium chloride Vetec(TM) reagent grade, >=98% Iodonitrotetrazolium chloride Vetec(TM) reagent grade, 95% INT CHLORIDE INT DYE INT IODONITROTETRAZOLIUM VIOLET INT~2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride hydrate Iodonitrotetrazolium chloride ,99% IODONITROTETRAZOLIUM CHLORIDERESEARCH GRADE Iodonitrotetrazolium chloride,2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, p-Iodonitrotetrazolium Violet, INT IntA.R. 2(4-IODOPHENYL)-3(4-NITROPHENYL)-5-PHENYL-2H-Tetrazolium Chloride (INT)extrapure AR p-Iodonitrotetrazolium Violet, INT, 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Iodonitrotetrazolium purple INT, Iodonitrotetrazolium violet 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride hydrate,99% 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium Chloride [for Biochemical Research] Iodonitrotetrazolium chloride p.a. INT, 4-Iodonitrotetrazolium violet INT Iodonitrotetrazolium Chloride p-Iodonitrotetrazolium Violet 2h-tetrazolium, 2-(piodophenyl)-3-(p-nitrophenyl)-5-phenyl-, Chloride 2-(p-iodophe IODONITRO TETRAZOLIUM (INT) P-IODONITROTETRAZOLIUM VIOLET 2H-TETRAZOLIUM, 2-(4-IODOPHENYL)-3-(4-NITROPHENYL)-5- PHENYL-, CHLORIDE 2-(P-IODOPHENYL)-3-(P-NITROPHENYL)-5-PHENYL-2H-TETRAZOLIUM CHLORIDE 2-(P-IODOPHENYL)-3-(P-NITROPHENYL)-5-PHENYLTETRAZOLIUM CHLORIDE 2-(4-IODOPHENYL)-3-(4-NITROPHENYL)-5-PHENYL-2H-TETRAZOLIUM CHLORIDE 2-(4'-IODOPHENYL)-3-(4''-NITROPHENYL)-5-PHENYLTETRAZOLIUM CHLORIDE 2-(4-IODOPHENYL)-3-(4-NITROPHENYL)-5-PHENYLTETRAZOLIUM CHLORIDE 4-IODONITROTETRAZOLIUM VIOLET 3-(P-IODOPHENYL)-2-(P-NITROPHENYL)-5-PHENYL TETRAZOLIUM CHLORIDE IODONITROTETRAZOLIUM IODONITROTETRAZOLIUM CHLORIDE 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2h-tetrazoliuchloride INT Iodonitrotetrazolium chloride 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazoliumChloride> 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride(INT) ,≥98% 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-ium chloride 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chlor... Iodonitrotetrazolium chloride, for cell culture, 98%+ Iodonitrotetrazolium chloride, for cell culture+ 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-3H-tetrazol-2-ium chloride IODONITROTETRAZOLIUM CHLORIDE (INT DYE) 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazol-2-ium:chloride 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-tetrazol-3-ium chloride Iodonitrotetrazolium chloride Ve 2-(p-Iodophenyl)-3-(p-nitrophenyl)-5-phenyl-2H-tetrazolium c 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazoliumChloride[forBiochemicalResearch]> lodonitrotetrazolium chloride odonitrotetrazolium chloride 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride(INT) I.N.T. 95% AR 146-68-9