Uses

Monoclonal Anti-RAP1 A/B antibody produced in mouse may be used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry

General Description

Monoclonal Anti-RAP1 A/B (mouse IgM isotype) is derived from the hybridoma R116 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice. Ras-related protein 1A (RAP1A/Krev-1) belongs to the Ras superfamily of small GTPases. RAP1 gene is mapped to human chromosome 1p13.3

Biochem/physiol Actions

Ras-related protein 1A (RAP1A), a novel oncoprotein in human malignancies acts as an important modulator in T cell response. RAP1A also participates in bacterial infection, cell dynamics and osteoblast differentiation. It may serve as a Ras antagonist and is a tumor suppressor gene. RAP1A is activated by growth factors, neurotransmitters and cytokines. Several second messengers, such as cyclic adenosine monophosphate (cAMP), diacylglycerol and calcium may serve as the mediators of this activation. In many cells, active RAP1 changes its cellular location from endosomal membranes to the plasma membrane. RAP1 binds competitively to RAS-GTPase activating protein (RASA) thus interfering with its RAS effectors functions. Ectopically expressed RAP1 is capable of interacting with the RAS effector (RAF1), which may lead to the inhibition of extra-cellular-signal-regulated kinase (ERK) activation. RAP1 can also mediate cyclic adenosine monophosphate (cAMP)-induced ERK activation.