Uses

Digoxigenin-11-UTP has been used for labeling RNA probes in:

• in situ hybridization
• in situ reverse transcriptase (RT)-polymerase chain reaction (PCR)
• fluorescence in situ hybridization (FISH)

General Description

Digoxigenin (DIG) -11-uridine triphosphate (UTP) is provided as a solution of the tetralithium salt in 3.5mM (200nmol) or 10mM (250nmol) concentration. It is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for DIG labeling of RNA in a ratio of 35:65%.

Biochem/physiol Actions

Linearized template DNA with T7, SP6, or T3 promoter can be in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and digoxigenin-11-UTP, respectively. The labeled RNA can be subsequently detected with the anti-Digoxigenin-AP (alkaline phosphatase), fab fragments, the digoxigenin nucleic acid detection kit, or the digoxigenin luminescent detection kit for nucleic acids.