931417-26-4
物理化學(xué)性質(zhì)
報價日期 | 產(chǎn)品編號 | 產(chǎn)品名稱 | CAS號 | 包裝 | 價格 |
2024/11/08 | HY-117102 | 化合物 T10325 ANI-7 | 931417-26-4 | 5mg | 700元 |
2024/11/08 | HY-117102 | 化合物 T10325 ANI-7 | 931417-26-4 | 10mM * 1mLin DMSO | 770元 |
2024/11/08 | HY-117102 | 化合物 T10325 ANI-7 | 931417-26-4 | 10mg | 1200元 |
常見問題列表
Aryl Hydrocarbon Receptor
|
Chk2
|
ANI-7 (2.5 μM; 24 hours; MCF10A and MDA-MB-468 cells) treatment induces significant S-phase and G2 + M-phase cell cycle arrest within 24 hours of treatment in MDA-MB-468 cells, and negligible effect in normal breast MCF10A cells.
ANI-7 (2 μM; 12-24 hours; MDA-MB-468 cells) treatment results in a significant increase in the content and phosphorylation of CHK2, and induces a significant increase in H2AX? in MDA-MB-468 cells, indicative of DNA double-strand damage.
Inhibition of the AhR pathway ameliorates the effects of ANI-7. ANI-7 activates XRE activity and expression of the AhR and CYP1 members.
Comparisons of the GI
50
values show that ANI-7 produces a GI
50
value of 0.38 μM in MCF-7 cells, whereas values of 3.0-42 μM are observed in cell lines from lung, colon, ovary, neuronal, glial, prostate, and pancreas. The only other tumor type that shows appreciable growth inhibition by ANI-7 is the A431 vulva cell line (GI
50
of 0.51μM).
ANI-7 potently inhibits the growth of T47D, ZR-75-1, MCF-7, SKBR3, and MDA-MB-468 breast cancer cells (GI
50
range of 0.16-0.38 μM), moderately inhibits the growth of BT20 and BT474 cells (GI50 range of 1-2 μM), and essentially fails to inhibit the growth of MDA-MB-231 and MCF10A cells (GI
50
range of 17-26 μM). Moreover, ANI-7 maintained its ability to inhibit the growth of drug-resistant cells (MCF-7/VP16: GI
50
of 0.21 μM).
Cell Cycle Analysis
Cell Line: | MCF10A and MDA-MB-468 cells |
Concentration: | 2.5 μM |
Incubation Time: | 24 hours |
Result: | Induced significant S-phase and G2 + M-phase cell cycle arrest in MDA-MB-468 cells, and negligible effect in normal breast MCF10A cells. |
Western Blot Analysis
Cell Line: | MDA-MB-468 cells |
Concentration: | 2 μM |
Incubation Time: | 12 hours, 24 hours |
Result: | Resulted in a significant increase in the content and phosphorylation of CHK2 (25-fold increase),and induced a significant increase in H2AX? (3.5-fold increase). |