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86636-92-2

中文名稱 6-丙烯?;?2-二甲氨基萘
英文名稱 6-ACRYLOYL-2-DIMETHYLAMINONAPHTHALENE
CAS 86636-92-2
分子式 C15H15NO
分子量 225.29
MOL 文件 86636-92-2.mol
更新日期 2023/06/30 11:21:13
86636-92-2 結(jié)構(gòu)式 86636-92-2 結(jié)構(gòu)式

基本信息

中文別名
6-丙烯酰-2-二甲氨基萘
6-丙烯?;?2-二甲氨基萘
1-(6-(二甲基氨基)萘-2-基)丙-2-烯-1-酮
英文別名
ACRYLODAN
6-ACRYLOYL-2-DIMETHYLAMINONAPHTHALENE
1-(6-Dimethylamino-naphthalen-2-yl)-propenone
Acrylodan [6-Acryloyl-2-dimethylaminophthalene]
ACRYLODAN [6-ACRYLOYL-2-DIMETHYLAMINONAPHTHALENE]
1-(6-(Dimethylamino)-2-naphthalenyl)-2-propen-1-one
1-(6-(Dimethylamino)naphthalen-2-yl)prop-2-en-1-one
2-Propen-1-one, 1-(6-(dimethylamino)-2-naphthalenyl)-
所屬類別
生物化工:熒光成像

物理化學(xué)性質(zhì)

熔點(diǎn)130-131 °C(Solv: ethanol (64-17-5); water (7732-18-5))
沸點(diǎn)388.3±24.0 °C(Predicted)
密度1.100±0.06 g/cm3(Predicted)
儲(chǔ)存條件Sealed in dry,2-8°C
酸度系數(shù)(pKa)4.32±0.40(Predicted)
形態(tài)Solid
顏色Light yellow to yellow

安全數(shù)據(jù)

危險(xiǎn)性符號(hào)(GHS)GHS hazard pictograms
GHS07
警示詞警告
危險(xiǎn)性描述H302-H315-H319-H335

常見問題列表

生物活性
Acrylodan 可與硫醇反應(yīng),對(duì) Cys34 周圍的結(jié)合口袋內(nèi)的局部環(huán)境雙極性和動(dòng)力學(xué)敏感。
體外研究

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Purification and Labeling of Act1:
1. 20-fold molar excess of acrylodan (Anaspec) was added to the polymerized actin and incubated on ice overnight.
2. The labeled actin is centrifuged (2 h, 4 °C, 100,000 × g) then the pellet was resuspended in 10 mm Tris, pH 7.5, 0.5 mm β-mercaptoethanol, 0.2 mm CaCl2, 50 μm ATP and dialyzed against the same buffer for 70 h at 4 °C.
3. The supernatant was collected and the actin concentration and degree of labeling were calculated using the extinction coefficient 26,600 M ?1 cm ?1 for actin at 290 nm, 18,500 M ?1 cm ?1 or acrylodan at 385 nm, and 71,000 M ?1 cm ?1 for Alexa Fluor 488 at 494 nm.
4. Acrylodan-labeled Act1 ( Acryl Act1 NAT ) is dramatically different than that of EDTA-unfolded Acryl Act1 ( Acryl Act1I3), which has a very similar emission spectrum to CCT-bound Acryl Act1.

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