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76421-73-3

中文名稱 CHLOROBIMANE
英文名稱 MONOCHLOROBIMANE
CAS 76421-73-3
分子式 C10H11ClN2O2
分子量 226.66
MOL 文件 76421-73-3.mol
更新日期 2025/01/08 11:39:05
76421-73-3 結(jié)構(gòu)式 76421-73-3 結(jié)構(gòu)式

基本信息

中文別名
單氯二胺
單氯二胺 MBCL
MBCL [單氯二胺]
英文別名
MCB
MBCL
CHLOROBIMANE
THIOLYTE(R) MC
MONOCHLOROBIMANE
THIOLYTE(R) MC REAGENT
MBCl [MonochlorobiMane]
THIOLYTE(R) MONOCHLOROBIMANE REAGENT
mBCl [Monochlorobimane] *CAS 76421-73-3*
THIOLYTE(R) MONOCHLOROBIMANE REAGENT, FLUORESCENT LABELING COMPOUND
所屬類別
有機(jī)原料:氨基化合物

物理化學(xué)性質(zhì)

熔點(diǎn)135-136 °C(lit.)
沸點(diǎn)314.9±44.0 °C(Predicted)
密度1.40±0.1 g/cm3(Predicted)
儲(chǔ)存條件Keep in dark place,Inert atmosphere,2-8°C
溶解度DMF: soluble
酸度系數(shù)(pKa)-3.22±0.70(Predicted)
形態(tài)Solid
顏色Light yellow to yellow
BRN4440901

安全數(shù)據(jù)

危險(xiǎn)性符號(hào)(GHS)GHS hazard pictograms
GHS07
警示詞警告
危險(xiǎn)性描述H315-H319-H335
危險(xiǎn)品標(biāo)志Xi
危險(xiǎn)類別碼36/37/38
安全說(shuō)明26-36
WGK Germany3
F8

常見(jiàn)問(wèn)題列表

生物活性
Monochlorobimane (Chlorobimane) 是一種熒光染料 (λex=380 nm,λem=470 nm),用于測(cè)定細(xì)胞中谷胱甘肽 (GSH)。
體外研究

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparation of control and experimental wells:
The experiment should consist of parallel negative, positive and experimental wells respectively.
Experimental wells: add 200 μL of cell culture media containing your GSH effector of interest at desired concentration (e.g. 200 μM H 2 O 2 )
2. Incubate the plate overnight at 37?°C, 5?% CO 2 . The incubation time varies depended on your normal protocol
3. Monochlorobimane (mBCl) is added to the cells at a final concentration of 20-100 μM from a working solution of 1 mM. The stock solution of mBCl (50 mM) was prepared in dimethyl sulphoxide (DMSO) and stored at -20°C; the working solution was prepared before use by diluting the stock solution in 0.1 M PBS buffer (pH 7.0). In the assay the final concentration of DMSO was below 0.2% (v/v)
4. The cell suspensions were incubated with mBCl in the dark at 25°C for ~2 h
5. Remove the dye and treatment by centrifugation at 700 X g for 5 min. Add 200 μL of the Assay Buffer to each well and continue to the preferred method of detection
6. Detection of intracellular GSH:
Fluorescence plate reader: For adherent cells; read fluorescence directly off the culturing plate. If working with suspension cells; aliquot 200 μL of the culture suspension into the white opaque plate and record fluorescence at E X /E M = 380/465 ±20 nm respectively ( blue ).

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