466-61-5
466-61-5 結(jié)構(gòu)式
基本信息
(15R)-15-Methyllycopodan-5-one
1,9-Ethanobenzo[i]quinolizin-14-one, dodecahydro-11-methyl-, (1S,8aR,9S,11R,12aR)-
物理化學(xué)性質(zhì)
常見(jiàn)問(wèn)題列表
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Caspase-3
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5-Lipoxygenase
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Lycopodine (5.22-78.3 μg/mL; 12 hours) has 50% viability at 57.62±0.086 μg/mL and 51.46±1.43 μg/mL for PC3 and LnCaP, respectively.
Treated with Lycopodine (74-222 mM; 12 hours), the apoptotic index is with respect to the gradual increase in doses for the PC3 and LnCaP cells.
Lycopodine (74-222 mM; 12 hours) induces cell cycle arrest at G0/G1 phase in PC3 and LnCaP cells.
Lycopodine (0-200 μg/mL; 48 hours) shows cytotoxicity to HeLa cells in a dose and time dependent manner. However, Lycopodine shows minimal cytotoxic effects in normal peripheral blood mononuclear cells (PBMC) even at the highest dose (200 μg/mL).
Lycopodine (100, 200 μg/mL; 24 hours) increases level of Bax and decreases the mitochondrial cytochrome c. This is followed by an increase in expression of cytochrome c in cytosolic fraction. Lycopodine also cleaves the caspase-3 in the total cell lysate, while the expression of Bcl-2 is down regulated.
Cell Viability Assay
| Cell Line: | PC3 and LnCaP cells |
| Concentration: | 5.22-78.3 μg/mL |
| Incubation Time: | 12 hours |
| Result: | Reached 50% viability at 57.62±0.086 μg/mL and 51.46±1.43 μg/mL for PC3 and LnCaP, respectively. |
Apoptosis Analysis
| Cell Line: | PC3 and LnCaP cells |
| Concentration: | 74, 148, 222 mM |
| Incubation Time: | 12 hours |
| Result: | The apoptotic index was with respect to the gradual increase in doses. |
Cell Cycle Analysis
| Cell Line: | PC3 and LnCaP cells |
| Concentration: | 74, 148, 222 mM |
| Incubation Time: | 12 hours |
| Result: | Arrested growth of the cells at G0/G1 phase in the case of PC3 and LnCaP cells. |
Cell Cytotoxicity Assay
| Cell Line: | Hela cells and PBMC |
| Concentration: | 0-200 μg/mL |
| Incubation Time: | 48 hours |
| Result: | A linear increase of the cytotoxicity was along with the increase of time of treatment and also of the dose. |
Western Blot Analysis
| Cell Line: | HeLa cells |
| Concentration: | 100, 200 μg/mL |
| Incubation Time: | 24 hours |
| Result: | Increased level of Bax and decreased the mitochondrial cytochrome c. |