42424-50-0
42424-50-0 結構式
基本信息
雙鏈聚肌胞溶液
雙鏈聚肌胞鈉鹽
聚肌苷酸-聚胞苷酸鈉鹽
聚肌苷 - 聚胞苷酸鈉鹽
卡那霉素-聚肌胞鈉鹽復合物
聚肌胞溶液(雙鏈聚肌胞溶液)
POLYI:C雙鏈聚肌胞鈉鹽
6.雙鏈聚肌胞(POLYI:C .)
Poly(I:C) sodium
POLY I POLY C, NA
Polymyocyte sodium salt
POLY[I] POLY[C] SODIUM SALT
polyinosinic-polycytidylic acid sodium
polyI-polyC sodium salt compound solution
POLYINOSINIC-POLYCYTIDYLIC ACID SODIUM SALT
POLYINOSINIC ACID POLYCYTIDYLIC ACID, SODIUM SALT
POLYINOSINIC ACID + POLYCYTIDYLIC ACID, SODIUM SALT
物理化學性質
常見問題列表
TLR3, RIG-I, MDA5, apoptosis
Polyinosinic-polycytidylic acid (20 ng/mL; 24 hours; WM793, WM278, WM239A, WM9 and 1205Lu cells) treatment strongly reduces viability from 100% in controls to 20%–50% within 24 hours.
Polyinosinic-polycytidylic acid (200 ng/mL; 24 hours; 1205Lu cells) treatment induces apoptosis in 1205Lu cells.
Polyinosinic-polycytidylic acid (3 ng/mL; 24 hours; 1205Lu cells) treatment induces IFN-β expression in melanoma cells. Silencing of RIG-I and MDA-5 confirmed that induction of IFN-β by Polyinosinic-polycytidylic acid required RIG-I and MDA-5, respectively, and that required IPS-1.
Polyinosinic-polycytidylic acid (5 ng/mL; 24 hours; 1205Lu cells) treatment reveals active subunits of caspase-9 and caspase-8 in melanoma cells.
Polyinosinic-polycytidylic acid sodium is prepared for injection by resuspending in sterile saline, heating to 50 °C at a concentration of 2 mg/ml to ensure complete solubilisation and then allowing to cool naturally to room temperature to ensure proper annealing of double-stranded RNA. Poly I:C is stored at -20 °C until use.
Cell Viability Assay
| Cell Line: | WM793, WM278, WM239A, WM9 and 1205Lu cells |
| Concentration: | 20 ng/mL |
| Incubation Time: | 24 hours |
| Result: | Strongly reduced viability from 100% in controls to 20%–50% within 24 hours. |
Apoptosis Analysis
| Cell Line: | 1205Lu cells |
| Concentration: | 200 ng/mL |
| Incubation Time: | 24 hours |
| Result: | Induced apoptosis in 1205Lu cells. |
RT-PCR
| Cell Line: | 1205Lu cells |
| Concentration: | 3 ng/mL |
| Incubation Time: | 24 hours |
| Result: | Induced IFN-β expression in melanoma cells. |
Western Blot Analysis
| Cell Line: | 1205Lu cells |
| Concentration: | 5 ng/mL |
| Incubation Time: | 24 hours |
| Result: | Revealed active subunits of caspase-9 and caspase-8 in melanoma cells. |
Polyinosinic-polycytidylic acid treatment inhibits tumor growth in NOD/SCID immunodeficient mice injected with 1205Lu cells. The level of human DNA is 50% lower in mice treated with Polyinosinic-polycytidylic acid.