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210292-65-2

中文名稱 NAPH-EA-MAL
英文名稱 1H-Benz[de]isoquinoline-1,3(2H)-dione, 2-butyl-6-[[2-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)ethyl]amino]-
CAS 210292-65-2
分子式 C22H21N3O4
分子量 391.42
MOL 文件 210292-65-2.mol
210292-65-2 結(jié)構(gòu)式 210292-65-2 結(jié)構(gòu)式

基本信息

英文別名
Naph-EA-mal
Thiol-green 1
1H-Benz[de]isoquinoline-1,3(2H)-dione, 2-butyl-6-[[2-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)ethyl]amino]-

物理化學(xué)性質(zhì)

沸點(diǎn)633.5±45.0 °C(Predicted)
密度1.371±0.06 g/cm3(Predicted)
酸度系數(shù)(pKa)1.51±0.20(Predicted)
形態(tài)Solid
顏色Light yellow to orange

常見問題列表

生物活性
Naph-EA-mal (Thiol-green 1) 是一種快速檢測和超快 turn-on 型硫醇 (thiol) 熒光探針，可用于蛋白標(biāo)記和生物成像。Naph-EA-mal (Thiol-green 1) 可用于檢測活細(xì)胞中的硫醇，標(biāo)記蛋白質(zhì)硫醇，定量細(xì)胞裂解液中總硫醇的濃度，并確定固定細(xì)胞中可逆的蛋白質(zhì)硫醇氧化。Ex: 488 nM; Em: 540 nM。
體外研究

Imaging thiols in living cellsHep G2 cells incubated with Naph-EA-mal (Thiol-green 1) (1 μM;5 mins), and strong green fluorescence can be observed. When cells are preincubated the cells to block the intracellular thiols with NEM (a thiol block reagent; 100 μM), a weaker fluorescence is observed in cells.Monitoring thiol-disulfide levels in fixed cellsDue to the majority of cellular thiols are present in the reduced form, Naph-EA-mal (Thiol-green 1) (10 μM) induces strong fluorescence in Hep G2 cells. After diamide treatment, fluorescent signal sharp dimed is observed.The Hep G2 cells, oxidized by diamide (200 μM; 30 min), are fixed by 70% EtOH and the remaining free thiols are blocked by NEM (100 μM). then The cells are further treated with TCEP (5 mM) followed by addition of the Naph-EA-mal (Thiol-green 1) (10 μM). The green fluorescent signal is detected again

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