1875036-74-0
1875036-74-0 結(jié)構(gòu)式
物理化學(xué)性質(zhì)
沸點(diǎn)632.6±55.0 °C(Predicted)
密度1.29±0.1 g/cm3(Predicted)
儲(chǔ)存條件-20°C儲(chǔ)存
溶解度DMSO : 125 mg/mL (293.08 mM; Need ultrasonic)
酸度系數(shù)(pKa)11.59±0.70(Predicted)
形態(tài)Solid
顏色White to off-white
常見問題列表
生物活性
BI8622 是一種泛素連接酶 HUWE1 特異性拮抗劑，IC50 為 3.1 μM。靶點(diǎn)
IC50: 3.1 μM (HUWE1)
體外研究
BI8622 induces HUWE1 ectopically expresses to abolish ubiquitination of MCL1 with an IC
50
value of 6.8 μM in HeLa cells.
BI8622 suppresses colony formation of Ls174T cells with estimated IC
50
value of 8.4 μM.
BI8622 (10 μM; 1-4 days) treatment retards passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1.
BI8622 (0-50 μM; 16 hours) retards the degradation of MCL1 in response to UV irradiation by inhibiting HUWE1 in HeLa cells.
BI8622 inhibits MYC-dependent transactivation in colorectal cancer cells.
Cell Cycle Analysis
| Cell Line: | Ls174T cells |
| Concentration: | 0 μM, 5 μM,10 μM, 15 μM, 20 μM |
| Incubation Time: | 0-4 days |
| Result: | Retarded passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1. |
Western Blot Analysis
| Cell Line: | HeLa cells |
| Concentration: | 0 μM, 10 μM, 20 μM |
| Incubation Time: | 16 hours |
| Result: | Retarded the degradation of MCL1 in response to UV irradiation by inhibiting HUWE1 in HeLa cells. |