150651-39-1
150651-39-1 結(jié)構(gòu)式
基本信息
1-氨基-3-(對甲苯基)苯并[4,5]咪唑并[1,2-A]吡啶-2,4-二甲腈
1-amino-3-(4-methylphenyl)pyrido[1,2-a]benzimidazole-2,4-dicarbonitrile
Pyrido[1,2-a]benzimidazole-2,4-dicarbonitrile, 1-amino-3-(4-methylphenyl)-
物理化學(xué)性質(zhì)
常見問題列表
| Target | Value |
|
FSP1/AIFM2
(Cell-free assay) | 103 nM(EC50) |
iFSP1 (0.001-1 μM; 24 hours) inhibits the Gpx4-knockout cell growth as a dose-dependent manner, but does not inhibit the wild type cell growth. Treatment with the ferroptosis inhibitor Lip-1 protects GPX4- knockout cells from iFSP1-induced ferroptosis.iFSP1 (0.001-1 μM; 24 hours) is less efficient than genetic deletion of?FSP1, whereas iFSP1 treatment in the?FSP1-knockout background had no additive effect to RSL3-induced?ferroptosis.iFSP1 (3 μM; 24 hours) treatment results in an obvious toxicity of RSL3 in a panel of genetically engineered (FSP1-knockout) human cancer cell lines.AIFM2: the flavoprotein apoptosis-inducing factor mitochondria-associated 2 is a previously unrecognized anti-ferroptotic gene. AIFM2, which is renamed ferroptosis suppressor protein 1 (FSP1)
Cell Proliferation Assay
| Cell Line: | Wild-type and?Gpx4-knockout Pfa1 or HT 1080 cells overexpressing FSP1–HA |
| Concentration: | 0.001-1 μM |
| Incubation Time: | 24 hours |
| Result: | Was toxic to cells that depend solely (no GPX4 expression detectable) on FSP1 function. |
Cell Viability Assay
| Cell Line: | NCl-H1437, NCl-H1437 FSP1 KO, U-373, U-373 FSP1 KO, MDA-MB-436, MDA-MB-436 FSP1 KO, SW620, SW620 FSP1 KO, MDA-MB-435S, MDA-MB-435S FSP1 KO, A549 and A549 FSP1 KO |
| Concentration: | 3 μM |
| Incubation Time: | 24 hours |
| Result: | Sensitized a variety of human cancer cell lines to the ferroptosis inducer (1S,3R)-RSL3. |