Identification | Back Directory | [Name]
EC 1.13.11.3 | [CAS]
9029-47-4 | [Synonyms]
EC 1.13.11.3 PROTOCATECHUATE 3,4-DIOXYGENASE protocatechuate 3,4-dioxygenase from*pseudomonas PROTOCATECHUATE 3,4-DIOXYGENASE FROM*PSE UDOMONAS SP Protocatechuate-3,4-dioxygenase,from Pserdomonas sp. Native Pseudomonas sp. Protocatechuate 3, 4-dioxygenase Protocatechuate 3,4-Dioxygenase from Bacteria, Recombinant rPCO, Protocatechuate: oxygen 3,4-oxidoreductase (decyclizing) Protocatechuate 3,4-Dioxygenase, recombinant from bacteria, solution frompseudomonasspecies,lyophilizedpowderactivity:3-5umgsolidcontainsapprox.40 | [MDL Number]
MFCD00132132 |
Chemical Properties | Back Directory | [storage temp. ]
-20°C | [form ]
lyophilized powder | [color ]
White to off-white | [biological source]
bacterial (Pseudomonas spp.) | [Specific Activity]
≥3units/mg solid |
Hazard Information | Back Directory | [Uses]
The enzyme has been used to create an oxygen scavenging system along with protocatechuate (PCA) and Trolox. The enzyme employs a nonheme iron center that catalyzes the conversion of PCA and molecular oxygen into β-carboxy-cis,cis-muconic acid, while the antioxidant Trolox suppresses slow blinking and photobleaching of cyanine dyes. It has been used in the preparation of imaging buffer along with DMB-BSA (dynein motility buffer-BSA), ATP and protocatechuate in single molecule motility assay. | [General Description]
Protocatechuate 3,4-Dioxygenase belongs to the non-heme iron family of enzymes. The active site of the enzyme contains Fe3+. | [Biochem/physiol Actions]
Protocatechuate 3,4-Dioxygenase catalyzes the degradation of 3,4-dihydroxybenzoate (protocatechuate) into β-carboxy-cis,cis-muconate. |
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