| Identification | Back Directory | [Name]
EC 4.1.3.3 | [CAS]
9027-60-5 | [Synonyms]
C:C&B4
EC 4.1.3.3
Sialate lyase
NANA Aldolase
SIALIC ALDOLASE
NEU5AC ALDOLASE
Sialic acid lyase
Neuraminic aldolase
Neu5Ac Aldolase 001
SIALIC ACID ALDOLASE
Neuraminate aldolase
N-Acylneuraminic lyase
Sialate pyruvate-lyase
Lyase,acetylneuraminate
N-Acylneuraminate lyase
Neuraminic acid aldolase
N-Acylneuraminic aldolase
N-Acylneuraminate aldolase
N-Acetylneuraminic Acid Lyase
N-Acylneuraminic acid aldolase
N-ACETYLNEURAMINIC ACID ALDOLASE
N-ACETYLNEURAMINIC ACID ALDORASE
N-ACETYLNEURAMINATE PYRUVATE LYASE
Sialic acid aldolase - crystalline
N-Acetylneuraminic Acid Aldolase，NAL
Sialic acid aldolase - Aqueous solution
N-Acetylneuraminic Acid Aldolase USP/EP/BP
Sialic Acid Aldolase from Escherichia coli K12
N-Acetylneuraminic acid Aldolase Escherichia coli
N-ACETYLNEURAMINIC ACID ALDOLASE >15 U/MG*
N-ACETYLNEURAMINIC ACID ALDOLASE FROMESC HERICHIA C
N-AcetylneuraMinic acid aldolase,froM MicroorganisMi
N-Acetylneuraminic Acid Aldolase from microorganisms
N-ACETYLNEURAMINIC ACID ALDOLASE, FROM MICROORGANISM
Native Microorganism N-Acetylneuraminic acid aldolase
n-acetylneuraminic acid aldolase from escherichia coli
Native Escherichia coli N-Acetylneuraminic Acid Aldolase
Sialic Acid Aldolase from Escherichia coli K12, Recombinant
N-Acetylneuraminate pyruvate-lyase (N-acetyl-D-mannosamine-forming)
N-Acetylneuraminate Pyruvate Lyase, N-Acetylneuraminic Acid Lyase, NANA Aldolase, Sialic Aldolase | [MDL Number]
MFCD00131656 |
| Hazard Information | Back Directory | [Uses]
Sialic acid aldolase can be used to synthesize unnatural sugars of C(6) to C(10) for the design of antagonists and inhibitors of glycoenzymes. | [Uses]
This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid when
coupled with the related enzymes in clinical analysis.
For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid. | [General Description]
Sialic acid aldolases, or N-acetylneuraminate lyases, catalyze the reversible aldol cleavage of N-acetylneuraminic acid to form pyruvate and N-acetyl-D-mannosamine. In nature, N-acetylneuraminate lyase mainly occurs in pathogens. | [Purification Methods]
Purify the aldolase by extraction with H2O, protamine precipitation, (NH4)2SO4 fractionation, Me2CO precipitation, acid treatment at pH 5.7 and precipitation at pH 4.5. The equilibrium constant for pyruvate + n-acetyl-D-mannosamine N-acetylneuraminidate at 37o is 0.64. The Km for N-acetylneuraminic acid is 3.9mM in phosphate at pH 7.2 and 37o. [Comb & Roseman Methods Enzymol 5 391 1962.] The enzyme from hog kidney (cortex) has been purified 1700-fold by extraction with H2O, protamine sulfate precipitation, (NH4)2SO4 fractionation, heating between 60-80o, a second (NH4)2SO4 fractionation and starch gel electrophoresis. The Km for N-acetylneuraminic acid is 1.5mM. [Brunetti et al. J Biol Chem 237 2447 1962.] |
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