| Identification | Back Directory | [Name]
Ribonuclease A | [CAS]
9001-99-4 | [Synonyms]
RP-1
RNASE
RNAase
RNASE A
S-RNase
Rnase I
Amphinase
DNase-free
EC 2.7.7.16
EC 3.1.4.22
EC 3.1.27.5
EC: 2.7.7.16
ribo-nucleas
RIBONUCLEASE
bonuclease A
IUB: 3.1.27.5
ribornuclease
RIBONUCLEASE B
RIBONUCLEASE A
RIBONUCLEASE I
RIBONUCLEASE S
Nuclease, ribo-
PANCREATIC RNASE
ALKALINE RIBONUCLEASE
ribonucleicphosphatase
RIBONUCLEASE I, E COLI
RIBONUCLEASE extrapure
Ribonuclease A-agarose
PANCREATIC RIBONUCLEASE
RIBONUCLEASE A TYPE I-A
RIBONUCLEASE A TYPE X-A
Ribonucleasebeefpancreas
RIBONUCLEASE A TYPE I-AS
RIBONUCLEASE A TYPE II-A
RNASE A, BOVINE PANCREAS
RIBONUCLEASE A TYPE III-A
RIBONUCLEASE A TYPE XII-A
ribonuclease S grade xii-S
RNase A Solution(100mg/ml)
RibonucleaseExBovinePancreas
RibonucleaseAExBovinePancreas
Ribonuclease A, Protease-Free
RibonucleaseDExBovinePancreas
RNase Molecular Biology grade
RNase A (from bovine pancreas)
RIBONUCLEASE B PROTEOMICS GRADE
ribonucleaseafrombovinepancreas
RIBONUCLEASE A, BOVINE PANCREAS
O-PHENYLENEDIAMINE REAGENT GRADE
WRIGHT'S STAIN CERTIFIABLE GRADE
Ribonuclease from Bovine Pancreas
RNASEA,LYOPHILIZEDPOWDER,HIGHPURITY
ribonuclease s from bovine pancreas
ribonuclease from aspergillus clavatus
TBS BUFFER 20X LIQUID ULTRA PURE GRADE
ribonuclease A solution molecular*biology
Ribonuclease B from bovine pancreas,RNase B
ribonuclease A type I-A from*bovine pancreas
ribonuclease A type I-as from*bovine pancreas
ribonuclease A type ii-A from*bovine pancreas
RIBONUCLEASE A FROM BOVINE PANCREAS, TYPE 1-A
Ribonuclease A froM bovine pancreas(Purified)
Ribonuclease A from bovine pancreas, lyophil.
RIBONUCLEASE A FROM BOVINE PANCREAS, ~90 U/MG
RIBONUCLEASE A FROM BOVINE PANCREAS, >70 U/MG*
ribonuclease A type xii-A from bovine*pancreas
ribonuclease A type iii-A from*bovine pancreas
RIBONUCLEASE A, WITH SCRAMBLED DISULFIDE BONDS
ribonuclease A type X-A: solution from*bovine pan
RIBONUCLEATE 3'-PYRIMIDINOOLIGONUCLEOTIDOHYDROLASE
RIBONUCLEATE 3-PYRIMIDINO-OLIGONUCLEOTIDOHYDROLASE
RIBONUCLEASE A SOLUTION MOLECULAR*BIOLOG Y REAGENT
rnase b glycoprotein standard from bovine pancreas
RIBONUCLEASE A, CHROMATOGRAPHICALLY*PURI FIED, MOLEC
RIBONUCLEASE A TYPE X-A: SOLUTION FROM*B OVINE PANCR
RIBONUCLEASE A FROM BOVINE PANCREAS, POW DER, ~80 U/MG
Ribonuclease A from bovine pancreas(Purified Solution)
RIBONUCLEASE A BOVINE PANCR.CA.70 KUNITZ-U/MG LYOPHIL.
RIBONUCLEASE A FROM BOVINE PANCREAS, POW DER, ~100 U/MG
RNase A, Protease-Free, Highly Purified, Bovine Pancreas
Ribonuclease A from bovine pancreas(DNase & Protease Free)
Ribonuclease A from bovine pancreas ca. 70 Kunitz units/mg
Ribonuclease A from bovine pancreas ca. 90 Kunitz units/mg
RIBONUCLEASE A FROM BOVINE PANCREAS, IN 0.2 M SO-PHOSPHATE*
RIBONUCLEASE A FROM BOVINE PANCREAS 4XCR SALTFREE ~70 U/MG
Ribonuclease A from bovine pancreas min. 80 Kunitz units/mg
Ribonuclease A from bovine pancreas min. 70 Kunitz units/mg
RIBONUCLEASE A FROM BOVINEPANCREAS CA.90 KUNITZ-U/MG LYOPHIL.
Ribonuclease A from bovine pancreas Vetec(TM) reagent grade, 80%
Ribonuclease A froM bovine pancreas Min. 70 Kunitz units/Mg lyophil.
Ribonuclease A froM bovine pancreas Min. 80 Kunitz units/Mg lyophil.
Ribonuclease I, Ribonucleate 3μ-pyrimidinooligonucleotidohydrolase
RibonucleaseA, with scrambled disulfide bonds from bovine pancreas
RNase B Glycoprotein Standard from bovine pancreas,RNase B, Ribonuclease B from bovine pancreas
Ribonuclease A from bovine pancreas
RNase I
RNase A
Ribonucleate 3′-pyrimidinooligonucleotidohydrolase
Ribonuclease I
Ribonuclease A from bovine pancreas,Pancreatic Ribonuclease, RNase A, Ribonuclease I, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase | [EINECS(EC#)]
232-646-6 | [Molecular Formula]
NULL | [MDL Number]
MFCD00132188 |
| Chemical Properties | Back Directory | [Appearance]
Lyophilized | [Melting point ]
243 °C (dec.) | [alpha ]
D25 (per mg N) -0.47° (c = 5) | [storage temp. ]
2-8°C
| [solubility ]
Reconstitute in 10mM acetate, pH 5.0 with or without 15mM calcium chloride | [form ]
solid
| [color ]
white
| [PH]
pH : 8.0 | [Water Solubility ]
Soluble in water. | [Sensitive ]
Moisture Sensitive | [EPA Substance Registry System]
Nuclease, ribo-(9001-99-4) |
| Hazard Information | Back Directory | [Chemical Properties]
Lyophilized | [Uses]
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA.Ribonuclease A from bovine pancreas has been used in a study to assess hybridase activity of human ribonuclease-1. Ribonuclease A from bovine pancreas has also been used in a study to investigate particle-based and monolithic columns for cation exchange protein displacement chromatography. | [General Description]
Human chromosome 14q11.2 shows the presence of all human RNases. Only eight (RNase 1-8) of them were true called as canonical RNases. And the rest nine to thirteen are called as noncanonical RNases. Human RNase 1 is called as HP-RNase or human pancreatic RNase. Human RNase 2 is also called as EDN (eosinophils derived neurotoxin) that is isolated from eosinophils. | [Biochem/physiol Actions]
Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. | [Purification Methods]
Purify it by (NH4)2SO4 fractionation, followed by PC cellulose chromatography and affinity chromatography (using Sepharose 4B to which (G)n is covalently bonded). [Schmukler et al. J Biol Chem 250 2206 1975.] Ribozymes. These are ribonucleic acids which act like protein enzyme in catalyzing the making and breaking of peptide bonds as well as catalyzing reactions and cleavage of DNA and RNA molecules. The short RNAs are being intensively studied (see RNAi below). MicroRNAs (miRNA, see also below) are ubiquitous and are also genetically produced. They are involved in numerous reactions from splicing RNA, e.g. mRNA (messenger RNA) to controlling transcription of DNA to RNA, and translation of RNA to protein. Each miRNA is capable of being involved in a small number to hundreds of interactions with nucleic acids and with proteins. |
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