| Identification | Back Directory | [Name]
GLUCOSE-6-PHOSPHATE DEHYDROGENASE | [CAS]
9001-40-5 | [Synonyms]
G-6-PD
G-6-P-DH
EC 1.1.1.49
EC: 1.1.1.49
IUB: 1.1.1.49
G-6-PDH TYPE V
G-6-PDH TYPE XV
G-6-PDH TYPE IX
G-6-pdh control
ZWISCHENFERMENT
G-6-PDH TYPE VII
G-6-PDH TYPE XV-B
ZWISCHENFERMENT G6P-DH
ZWISCHENFERMENT TYPE V
G-6-PDH-BIOTIN LABELED
GGTI 298 HYDROCHLORIDE
ZWISCHENFERMENT TYPE IX
ZWISCHENFERMENT TYPE XV
ZWISCHENFERMENT TYPE VII
ZWISCHENFERMENT TYPE XV-B
G-6-P-DH, Zwischenferment
6-Phosphoglucose dehydrogenase
Dehydrogenase,glucose6-phosphate
GLUCOSE-6-PHOSPHATE DEHYDROGENASE
fromyeastsaltfreepowder400unitsmg
D-Glucose 6-phosphate dehydrogenase
NADP-glucose-6-phosphate dehydrogenase
Glucose-6-Phosphate Dehydrogenase,G6PD
glicose-6-phosphate dehydrogenase, yeast
Glucose-6-phosphoric acid dehydrogenase
GLUCOSE-6-PHOSPHATE DEHYDROGENASE BAKE&
GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE V
GLUCOSE-6-PHOSPHATE DEHYDROGENASE FROM &
GLUCOSE-6-PHOSPHATE DEHYDROGENASE TORU&
GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE XV
GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE IX
G6P-DH(Glucose-6-Phosphate Dehydrogenase)
GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE VII
GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE XV-B
GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE XXIV
GLUCOSE-6-PHOSPHATE DEHYDROGENASE*TYPE X IX
GLUCOSE-6-PHOSPHATE DEHYDROGENASE extrapure
GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE XXIII
D-GLUCOSE-6-PHOSPHATE: NADP 1-OXIDOREDUCTASE
D-GLUCOSE-6-PHOSPHATE: NAD(P)+ 1-OXIDOREDUCTASE
Glucose-6-phosphate Dehydrogenase from yeast
Glucose-6-phosphate Dehydrogenase, 500 units/Mg
6-PGDH (L.M.), Glucose-6-phosphate Dehydrogenase
GLUCOSE-6-PHOSPHATE DEHYDROGENASE*CONTROL NORMAL
Glucose-6-phosphate 1-dehydrogenase (from Yeast)
Glucose-6-phosphate dehydrogenase [400 units/mg]
GLUCOSE-6-PHOSPHATE DEHYDROGENASE*CONTROL DEFICIENT
Glucose-6-phosphate Dehydrogenase from torula yeast
GLUCOSE-6-PHOSPHATE DEHYDROGENASE*TYPE I X FROM BAKE
GLUCOSE-6-PHOSPHATE DEHYDROGENASE*TYPE X I FROM TORU
GLUCOSE 6-PHOSPHATE DEHYDROGENASE,*LEUCO NOSTOC, REC
GLUCOSE-6-PHOSPHATE DEHYDROGENASE*TYPE X V FROM BAKE
GLUCOSE-6-PHOSPHATE DEHYDROGENASE*TYPE V II FROM BAK
G-6-PDH, D-Glucose-6-phosphate: NADP 1-oxidoreductade
glucose-6-phosphatedehydrogenasefromyeastsusp~120u/mg
G-6-PDH, D-Glucose-6-phosphate: NADP 1-oxidoreductase
G-3-Pdh(Glycerol-3-PhosphateDehydrogenase),Mesenteroides
Glucose-6-phosphate dehydrogenase [>= 150 units/mg solid]
GLUCOSE-6-PHOSPHATE DEHYDROGENASE FROM L EUCO.MES. ~150U/MG
GLUCOSE-6-PHOSPHATE DEHYDROGENASE, TORU-LA YEAST, ~180 U/MG
GLUCOSE-6-PHOSPHATE DEHYDROGENASE, BAKER 'S YEAST, ~240 U/MG
GLUCOSE-6-PHOSPHATE DEHYDROGENASE, LEUCONOSTOC MESENTEROIDES
Glucose-6-Phosphate Dehydrogenase,from Yeast, 500U/mg protein
Glucose-6-phosphate dehydrogenase from yeast ca. 140 U/mg protein
Glucose-6-phosphate.dehydrogenase from Leuconostoc mesenteroides
glucose-6-phosphate dehydrogenase from baker's yeast (s. cerevisiae)
Glucose-6-phosphate 1-dehydrogenase (from Leuconostoc mesenteroides)
Glucose-6-phosphate Dehydrogenase from Bacillus stearothermophilus
Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides,G-6-P-DH
Glucose-6-phosphate Dehydrogenase Biotin Labeled from baker's yeast
Glucose-6-phosphate Dehydrogenase froM Leuconostoc Mesenteroides(Lyophilized)
Phosphogluconate dehydrogenase (decarboxylating), ammonium sulfate suspension
Phosphogluconate dehydrogenase (decarboxylating), ammonium sulphate suspension
Glucose-6-phosphate Dehydrogenase-Agarose from baker's yeast (S.cerevisiae)
Glucose-6-phosphate 1-dehydrogenase, recombinant (from Leuconostoc mesenteroides)
Glucose-6-phosphate Dehydrogenase from baker's yeast (S. cerevisiae),G-6-P-DH, Zwischenferment | [EINECS(EC#)]
232-602-6 | [Molecular Formula]
n.a. | [MDL Number]
MFCD00081656 |
| Hazard Information | Back Directory | [Chemical Properties]
White to yellow powder | [Uses]
Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides has been used along with hexokinase in the determination of glucose from mice liver samples. | [Uses]
Glucose-6-phosphate dehydrogenase has been used in a study to investigate the effect of dehydroepiandrosterone on cell growth and mitochondrial function in TM-3 cells. Glucose-6-phosphate dehydrogenase has also been used in a study to investigate the incidence of apoptosis and transcript abundance in bovine follicular cells. Glucose-6-phosphate dehydrogenase is used to test ketose reductase activity in developing maize endosperm. | [Uses]
Glucose-6-phosphate dehydrogenase, Leuconostoc mesenteroides is used to convert p-glucose-6-phosphate to p-glucono-d-lactone-6-phosphate in presence of NADP+ or NAD+. It is used as an intermediate, chemical and research purpose and also used fo analysis. | [General Description]
Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration). | [Biochem/physiol Actions]
Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway<<<17>>>. | [Purification Methods]
The enzyme is useful for measuring pyridine nucleotides in enzyme recycling. The enzyme from Baker's yeast has been purified by (NH4)2SO4 fractionation, Me2CO precipitation, a second (NH4)2SO4 fractionation, concentration by DEAE-SF chromatography, a third (NH4)2SO4 fractionation and recrystallisation. Crystallisation is induced by addition of its coenzyme NADP, which in its presence causes rapid separation of crystals at (NH4)2SO4 concentration much below that required to precipitate the amorphous enzyme. To recrystallise, the crystals are dissolved in 0.01M NADP (pH 7.3) with (NH4)2SO4 at 0.55 saturation, and the crystals appear within 10 to 60minutes. After standing for 2-3days (at 4o) the (NH4)2SO4 is increased to 0.60 of saturation, and more than 80% of the activity in the original crystals is recovered in the fresh crystals. [Noltmann et al. J Biol Chem 236 1255 1961]. Large amounts can be obtained from rat livers. The livers are extracted with 0.025M phosphate buffer (pH 7.5) and precipitated with 3M (NH4)2SO4 (70% of activity). The precipitate is dissolved in 3volumes of 0.025M phosphate (pH 7.5), dialysed against this buffer + 0.2mM EDTA at 4o for 5hours, then diluted to 1% protein and the nucleic acids are precipitated by addition of 0.4volumes of 1% protamine sulfate. (NH4)2SO4 is added to a concentration of 2M (pH adjusted to 7.0 with NH3), the precipitate is discarded and the supernatant is adjusted to 2.8M (NH4)2SO4, dialysed, and the protein is adjusted to 1% and treated with Ca3(PO4)2 gel. The gel is added in three steps (1.5mL of 0.4% gel/mL per step), and the gel is removed by centrifugation after each addition. The third gel adsorbed 50% of the activity. The gel is eluted with 0.2M phosphate buffer (pH 7.4, 40mL/g of gel; 60% recovery). The extract is precipitated in 3volumes of (NH4)2SO4 (adjusted to 4M) to give enzyme with an activity of 30Wmoles/mg of protein per hour. [Lowry et al. J Biol Chem 236 2746 1961.] The Km values for the yeast enzyme are 20WM for G-6P and 2WM for NADP (Tris pH 8.0, 10-2 M MgCl2, 38o) [Noltmann & Kuby The Enzymes VII 223 1963]. |
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