| Identification | Back Directory | [Name]
5(6)-Carboxyfluorescein | [CAS]
72088-94-9 | [Synonyms]
5(6)-FAM
5(6)-Carboxyfluoresc
5(6)-CARBOXYLUORESCEIN
5(6)-CARBOXYFLUORESCEIN
4(5)-CARBOXYFLUORESCEIN
4(5)-Carboxyfluoresceine]
5(6)-Carboxyfluoresceine]
Fluoresceincarboxylic Acid
5(6)-Carboxyfluorescein 5GR
5(6)-CARBOXYFLUORESCEIN 99%
5-(AND-6)-CARBOXYFLUORESCEIN
5(6)-CARBOXYFLUORESCEIN 95+%
5(6)-Carboxy-fluorescein, isomer mixture
5(6)-CARBOXYFLUORESCEIN, FOR FLUORESCENC E
5(6)-Carboxyfluorescein, mixture of isomers, 97%
3',6'-Dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5(6)-carboxylic acid
9’-[9h]xanthene]-ar-carboxylicacid,3’,6’-dihydroxy-spiro[isobenzofuran-1(3h
3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid
Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid, 3',6'-dihydroxy-3-oxo- | [EINECS(EC#)]
276-331-1 | [Molecular Formula]
C42H24O14 | [MDL Number]
MFCD00151081 | [MOL File]
72088-94-9.mol | [Molecular Weight]
752.63 |
| Chemical Properties | Back Directory | [Appearance]
Dark-Orange to Brown Solid | [Melting point ]
275 °C
| [Boiling point ]
464.9°C (rough estimate) | [density ]
1.3418 (rough estimate) | [refractive index ]
1.6380 (estimate) | [storage temp. ]
Store at 2-8 | [solubility ]
DMSO (Sparingly), Methanol (Slightly, Heated) | [form ]
Solid | [color ]
Light Yellow to Dark Brown | [Water Solubility ]
slightly soluble | [InChIKey]
CPRVUBZYHZGASQ-UHFFFAOYSA-N | [SMILES]
C12(OC(=O)C3C=CC(C(=O)O)=CC1=3)C1=CC=C(O)C=C1OC1C=C(O)C=CC2=1.C12(OC(=O)C3C=C(C(=O)O)C=CC1=3)C1=CC=C(O)C=C1OC1C=C(O)C=CC2=1 | [EPA Substance Registry System]
Spiro[isobenzofuran- 1(3H),9'-[9H]xanthene]- ar-carboxylic acid, 3',6'-dihydroxy- 3-oxo-(72088-94-9) |
| Hazard Information | Back Directory | [Chemical Properties]
Dark-Orange to Brown Solid | [Uses]
A popular probe of determination of intracellular pH. | [Uses]
A useful reagent for the preparation of hydrolytically stable fluorescent conjugates and is a useful starting material for the synthesis of other fluorescein-derives reagent | [Description]
5(6)-Carboxyfluorescein is a mixture of 5-carboxy and 6-carboxy derivatives of fluorescein. It is commonly used to label biomolecules through a reaction involving the carboxyl group.1 Unlike its succinimidyl ester derivative CFSE (Item No. 14456), 5(6)-carboxyfluorescein is membrane impermeant. As a result, it can be used in studies of membrane permeability.2 5(6)-Carboxyfluorescein displays excellent fluorescence (excitation/emission at 492/514 nm, respectively), and its excitation maximum closely matches the 488 nm spectral line of argon-ion lasers. | [Definition]
ChEBI: 5(6)-carboxyfluorescein is a commercially available mixture of 5-carboxyfluorescein and 6-carboxyfluorescein isomers. A fluorescent dye, it is commonly used as a tracer agent. It has a role as a tracer and a fluorescent dye. It contains a 5-carboxyfluorescein and a 6-carboxyfluorescein. | [Solubility in organics]
5(6)-Carboxyfluorescein is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide. The solubility of 5(6)-carboxyfluorescein in these solvents is approximately 5, 0.5, and 1 mg/ml, respectively. |
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