Identification | Back Directory | [Name]
BROMOSULFALEIN | [CAS]
71-67-0 | [Synonyms]
bsp cbsp bsfsimes hepartest bspsodium bromsulfan bromthalein bromotaleina bromsulfalein bromsulphalein hepatestabrome BROMOSULFALEIN Bromosulfaleine hepatosulfalein hepartestabrome bromsulfthalein brom-tetragnost bromsulphthalein BROMO SULPHALEIN bromosulphthalein bromsulfophthalein sulfobromphthalein BROMOSULFOPHTHALEIN SULFOBROMOPHTHALEIN BROMOSULFONPHTHALEIN tetrabromsulfthalein sulphobromophthalein sodiumbromsulphalein sodiumbromosulfalein bromsulphaleinrsodium Bromsulphalein sodium sodiumbromsulphthalein tetrabromosulfophthalein sulfonicacid]disodiumsalt sodiumbromosulfophthalein sulphobromophthaleinsodium sodiumsulphobromophthalein SODIUM SULFOBROMOPHTHALEIN bromosulfophthalein sodium disodiumbromosulfophthalein BROMOSULPHALEIN DISODIUM SALT BROMOSULFOPHTHALEIN SODIUM SALT SULFOBROMOPHTHALEIN SODIUM SALT sodiumphenoltetrabromophthalein SULFOBROMOPHTHALEIN DISODIUM SALT phenoltetrabromophthaleinsulfonate BROMSULPHTHALEIN-GLUTATHIONECONJUGATE disodiumphenoltetrabromophthaleinsulfonate SULFOBROMOPHTHALEIN, DISODIUM SALT USP XXII disodium phenoltetrabromophthaleindisulphonate 4,5,6,7-tetrabromo-3’,3’’-disulfo-phenolphthaleidisodiumsalt PHENOLTETRABROMOPHTHALEIN-3',3''-DISULFONIC ACID DISODIUM SALT 3,4,5,6-Tetrabromophenolphthalein-3,'3"-disulfonic acid disodium salt
benzenesulfonicacid,3,3’-(4,5,6,7-tetrabromo-3-oxo-1(3h)-isobenzofuranylidene 5,5’-(4,5,6,7-tetrabromo-3-oxo-1(3h)-isobenzofuranylidene)bis[2-hydroxybenzene 3,3'-(4,5,6,7-Tetrabromo-3-oxoisobenzofuran-1-ylidene)bis(6-hydroxybenzenesulfonic acid sodium) Sulfobromophthalein disodium salt, Phenoltetrabromophthalein-3μ,3-disulfonic acid disodium salt disodium 6,6-dihydroxy-3,3-(4,5,6,7-tetrabromo-1,3-dihydro-3-oxoisobenzofuran-1-ylidene)dibenzenesulphonate disodium 6,6'-dihydroxy-3,3'-(4,5,6,7-tetrabromo-1,3-dihydro-3-oxoisobenzofuran-1-ylidene)dibenzenesulphonate Benzenesulfonic acid, 3,3'-(4,5,6,7-tetrabromo- 3-oxo-1(3H)-isobenzofuranylidene)bis[6-hydroxy-, disodium salt | [EINECS(EC#)]
200-761-0 | [Molecular Formula]
C20H8Br4Na2O10S2 | [MDL Number]
MFCD00012442 | [MOL File]
71-67-0.mol | [Molecular Weight]
838 |
Hazard Information | Back Directory | [Chemical Properties]
white crystalline solid | [Definition]
ChEBI: An organic sodium salt that is the disodium salt of bromosulfophthalein. | [Uses]
Sulfobromophthalein sodium salt hydrate is a reagent for the quantitative determination of proteins, a dye for liver diagnostics in vitro. | [Purification Methods]
Purify it by TLC on silica Gel G (Merck 250a particle size) in two solvent systems (BuOH/AcOH/H2O 30:7.5:12.5 v/v, and BuOH/propionic acid/H2O 30:20:7.5 v/v). When the solvent reaches a height of ~10cm, the plate is removed, dried in air and developed with NH3 vapour giving blue-coloured spots. Also, the dye can be chromatographed on MN Silica Gel with t-BuOH/H2O/n-BuOH (32:10:5 v/v) as eluent and visualised with a dilute KOH (or NaOH if the Na salt is required) spray. The product corresponding to bromosulfalein is scraped off and eluted with H2O, filtered and evaporated to dryness in a vacuum. It was dissolved in H2O and filtered through Sephadex G-25 and evaporated to dryness. [UV and IR identification: Barbier & DeVeerdt J Pharm Sci 57 819 1968, NMR: Kato et al. Chem Pharm Bull Jpn 20 581 1972, McGuire Anal Biochem 83 75 1977, Beilstein 18/9 V 461.] |
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