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ChemicalBook--->CAS DataBase List--->71-67-0

71-67-0

71-67-0 Structure

71-67-0 Structure
IdentificationBack Directory
[Name]

BROMOSULFALEIN
[CAS]

71-67-0
[Synonyms]

bsp
cbsp
bsfsimes
hepartest
bspsodium
bromsulfan
bromthalein
bromotaleina
bromsulfalein
bromsulphalein
hepatestabrome
BROMOSULFALEIN
Bromosulfaleine
hepatosulfalein
hepartestabrome
bromsulfthalein
brom-tetragnost
bromsulphthalein
BROMO SULPHALEIN
bromosulphthalein
bromsulfophthalein
sulfobromphthalein
BROMOSULFOPHTHALEIN
SULFOBROMOPHTHALEIN
BROMOSULFONPHTHALEIN
tetrabromsulfthalein
sulphobromophthalein
sodiumbromsulphalein
sodiumbromosulfalein
bromsulphaleinrsodium
Bromsulphalein sodium
sodiumbromsulphthalein
tetrabromosulfophthalein
sulfonicacid]disodiumsalt
sodiumbromosulfophthalein
sulphobromophthaleinsodium
sodiumsulphobromophthalein
SODIUM SULFOBROMOPHTHALEIN
bromosulfophthalein sodium
disodiumbromosulfophthalein
BROMOSULPHALEIN DISODIUM SALT
BROMOSULFOPHTHALEIN SODIUM SALT
SULFOBROMOPHTHALEIN SODIUM SALT
sodiumphenoltetrabromophthalein
SULFOBROMOPHTHALEIN DISODIUM SALT
phenoltetrabromophthaleinsulfonate
BROMSULPHTHALEIN-GLUTATHIONECONJUGATE
disodiumphenoltetrabromophthaleinsulfonate
SULFOBROMOPHTHALEIN, DISODIUM SALT USP XXII
disodium phenoltetrabromophthaleindisulphonate
4,5,6,7-tetrabromo-3’,3’’-disulfo-phenolphthaleidisodiumsalt
PHENOLTETRABROMOPHTHALEIN-3',3''-DISULFONIC ACID DISODIUM SALT
3,4,5,6-Tetrabromophenolphthalein-3,'3"-disulfonic acid disodium salt
benzenesulfonicacid,3,3’-(4,5,6,7-tetrabromo-3-oxo-1(3h)-isobenzofuranylidene
5,5’-(4,5,6,7-tetrabromo-3-oxo-1(3h)-isobenzofuranylidene)bis[2-hydroxybenzene
3,3'-(4,5,6,7-Tetrabromo-3-oxoisobenzofuran-1-ylidene)bis(6-hydroxybenzenesulfonic acid sodium)
Sulfobromophthalein disodium salt, Phenoltetrabromophthalein-3μ,3-disulfonic acid disodium salt
disodium 6,6-dihydroxy-3,3-(4,5,6,7-tetrabromo-1,3-dihydro-3-oxoisobenzofuran-1-ylidene)dibenzenesulphonate
disodium 6,6'-dihydroxy-3,3'-(4,5,6,7-tetrabromo-1,3-dihydro-3-oxoisobenzofuran-1-ylidene)dibenzenesulphonate
Benzenesulfonic acid, 3,3'-(4,5,6,7-tetrabromo- 3-oxo-1(3H)-isobenzofuranylidene)bis[6-hydroxy-, disodium salt
[EINECS(EC#)]

200-761-0
[Molecular Formula]

C20H8Br4Na2O10S2
[MDL Number]

MFCD00012442
[MOL File]

71-67-0.mol
[Molecular Weight]

838
Chemical PropertiesBack Directory
[Appearance]

white crystalline solid
[solubility ]

DMF: 5 mg/ml; DMSO: 5 mg/ml; Ethanol: slightly soluble; PBS (pH 7.2): 1 mg/ml
[form ]

Powder
[color ]

White to off-white
[Stability:]

Stable. Incompatible with strong oxidizing agents. May be air or moisture sensitive.
[Water Solubility ]

Soluble in water.
[Sensitive ]

Moisture Sensitive
[Merck ]

14,8954
[EPA Substance Registry System]

Benzenesulfonic acid, 3,3'-(4,5,6,7-tetrabromo-3-oxo-1(3H)-isobenzofuranylidene)bis[6-hydroxy-, disodium salt (71-67-0)
Hazard InformationBack Directory
[Chemical Properties]

white crystalline solid
[Definition]

ChEBI: An organic sodium salt that is the disodium salt of bromosulfophthalein.
[Uses]

Sulfobromophthalein sodium salt hydrate is a reagent for the quantitative determination of proteins, a dye for liver diagnostics in vitro.
[Purification Methods]

Purify it by TLC on silica Gel G (Merck 250a particle size) in two solvent systems (BuOH/AcOH/H2O 30:7.5:12.5 v/v, and BuOH/propionic acid/H2O 30:20:7.5 v/v). When the solvent reaches a height of ~10cm, the plate is removed, dried in air and developed with NH3 vapour giving blue-coloured spots. Also, the dye can be chromatographed on MN Silica Gel with t-BuOH/H2O/n-BuOH (32:10:5 v/v) as eluent and visualised with a dilute KOH (or NaOH if the Na salt is required) spray. The product corresponding to bromosulfalein is scraped off and eluted with H2O, filtered and evaporated to dryness in a vacuum. It was dissolved in H2O and filtered through Sephadex G-25 and evaporated to dryness. [UV and IR identification: Barbier & DeVeerdt J Pharm Sci 57 819 1968, NMR: Kato et al. Chem Pharm Bull Jpn 20 581 1972, McGuire Anal Biochem 83 75 1977, Beilstein 18/9 V 461.]
Safety DataBack Directory
[Hazard Codes ]

Xn
[Risk Statements ]

42/43
[Safety Statements ]

22-36/37
[WGK Germany ]

3
[RTECS ]

SM8395000
[TSCA ]

Yes
[Hazardous Substances Data]

71-67-0(Hazardous Substances Data)
Spectrum DetailBack Directory
[Spectrum Detail]

BROMOSULFALEIN(71-67-0)1HNMR
BROMOSULFALEIN(71-67-0)13CNMR
BROMOSULFALEIN(71-67-0)IR1
BROMOSULFALEIN(71-67-0)IR2
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