| Identification | Back Directory | [Name]
KERATANASE | [CAS]
55072-01-0 | [Synonyms]
KERATANASE
EC 3.2.1.103
ENDO-BETA-GALACTOSIDASE
β-Galactosidase bacterial
KERATANASE, PSEUDOMONAS SP.
KERATANASE, PSEUDOMONAS SPECIES
keratanase from pseudomonas sp.
Native Pseudomonas sp. Keratanase
keratan-sulfate endo-1,4-β-galactosidase
Endo-b-galactosidase, Escherichia freundii
Endo-β-galactosidase from Bacteroides fragilis
keratanase from pseudomonas species*10 units per
Keratanase, Pseudomonas sp. *Not for sale in Japan*
KERATANASE FROM PSEUDOMONAS SPECIES*10 U NITS PER VI
Endo-β-galactosidase from Bacteroides fragilis, Recombinant | [MDL Number]
MFCD00131433 |
| Hazard Information | Back Directory | [Uses]
Endo-β-galactosidase was used in fractional protein isolation. It was used for deglycosylation in glycoproteomics of the endothelial secretome of human endothelial cells. | [Biochem/physiol Actions]
Internal β(1-4) galactose linkages in unbranched, repeating poly-Nacetyllactosamine [GlcNAc β(1-3)Gal β (1-4)] structures are the preferred substrate.Sulfated structures such as keratan sulfate are also cleaved. Branching and/or fucosylation of the substrate may reduce or completely inhibit cleavage. Sulfation of C-6 on galactose will block cleavage. Oligosaccharides of the neolacto-group are cleaved at greatly reduced rates depending on the deviation from the preferred substrate. For example, Gal β(1-3)GlcNAc β(1-3) Gal β(1-4)Glc is cleaved at 5X10-5 the rate of keratan sulfateβ-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop. |
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Creative Enzymes
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1-516-855-7709 |
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www.creative-enzymes.com |
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SIGMA-RBI
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800 736 3690 (Orders) |
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www.sigma-aldrich.com |
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