| Identification | Back Directory | [Name]
Elastase | [CAS]
39445-21-1 | [Synonyms]
Elastase
Elastasee
EC 3.4.21.36
EC 3.4.21.37
Elastase, Purified
LYSOSOMAL ELASTASE
ELASTASE, LEUKOCYTE
Elastase USP/EP/BP
PANCREATOPEPTIDASE E
Elastase (pig pancreas)
Native Porcine Elastase
expigpancreas,freezedried
Elastase (swine pancreas)
Elastase from hog pancreas
ELASTASE, HUMAN NEUTROPHIL
ELASTASE, HUMAN NEUTROPHILS
ELASTASE, NEUTROPHIL, HUMAN
Elastase(swine pancreas) (9CI)
ELASTASE, FROM PORCINE PANCREAS
ElastaseExPigPancreas(FreezeDried)
ELASTASE FROM HOG PANCREAS, ~3 U/MG
ELASTASE FROM HOG PANCREAS, ~8 U/MG
ELASTASE FROM HOG PANCREAS, ~15 U/MG
LEUKOCYTE ELASTASE, HUMAN NEUTROPHILS
Elastase, pancreatic froM porcine pan
ElastaseExPigPancreas(Pre-Crystalline)
ELASTASE TYPE IV FROM PORCINE PANCREAS
Elastase from porcine pancreas(Purified)
Elastase from porcine pancreas(Suspension)
Elastase, pancreatic from porcine pancreas
Elastase froM porcine pancreas(Lyophilized)
ELASTASE, PANCREATIC TYPE I FROM*PORCINE PANCREAS
ELASTASE FROM HOG PANCREAS, CRYST. SUSP., 1-2 U/MG*
ELASTASE, PANCREATIC FROM PORCINE*PANCRE AS CELL CUL
ELASTASE, PANCREATIC TYPE II-A FROM*PORC INE PANCREA
ELASTASE, PANCREATIC TYPE III FROM*PORCI NE PANCREAS
Elastase from hog pancreas, Pancreatopeptidase E
Elastase, Porcine Pancreas, High Purity, Crystallized
Elastase, pancreatic from porcine pancreas, 30 units/mg
ELASTASEPORCINE PANCREAS (WHITE POWDER) 30-100 UNITS/MG.
Elastase froM porcine pancreas Min. 200 U/Mg lyophil. salt-free
Elastase from porcine pancreas lyophilized, powder, white, ~8 U/mg
Elastase from porcine pancreas,Elastase from hog pancreas, Pancreatopeptidase E | [EINECS(EC#)]
254-453-6 | [Molecular Formula]
NULL | [MDL Number]
MFCD00130997 |
| Chemical Properties | Back Directory | [RTECS ]
TS5052000 | [storage temp. ]
−20°C
| [solubility ]
H2O: soluble
| [form ]
lyophilized powder
| [color ]
white
| [PH]
8 | [biological source]
Porcine pancreas | [Water Solubility ]
Soluble in water. | [Specific Activity]
≥4units/mg protein |
| Hazard Information | Back Directory | [Uses]
Elastase is used in combination with other proteases for protein analysis by mass spectrometry. Elastase, is used in tissue dissociation because elastin is found in highest concentrations in the elastic fibers of connective tissues, elastase is frequently used to dissociate tissues that contain extensive intercellular fiber networks. It is usually used with other enzymes such as collagenase, trypsin, and chymotrypsin, membrane protein solubilization, protein sequence studies. | [General Description]
Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin. | [Biochem/physiol Actions]
Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9 kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. The pH optimum is found to be 8.0-8.5. It does not require any activator, but it is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, α2-macroglobulin, α1-antitrypsin, sulfonyl fluorides and p-dinitrophenyl diethylphosphate and high salt concentrations. It is extensively used in tissue and cell dissociation procedures. Elastase is effective in the isolation of Type II lung cells. |
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