| Identification | Back Directory | [Name]
APF | [CAS]
359010-70-1 | [Synonyms]
APF
AMINOPHENYL FLUORESCEIN
2-[3-(4-aminophenoxy)-6-oxoxanthen-9-yl]benzoic acid
2-[6-(4'-AMINO)PHENOXY-3H-XANTHEN-3-ON-9-YL]BENZOIC ACID
2-[6-(4-AMINOPHENOXY)-3-OXO-3H-XANTHEN-9-YL]-BENZOIC ACID
3'-(4-aminophenoxy)-6'-hydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one | [Molecular Formula]
C26H17NO5 | [MDL Number]
MFCD08062220 | [MOL File]
359010-70-1.mol | [Molecular Weight]
423.42 |
| Chemical Properties | Back Directory | [Boiling point ]
677.7±55.0 °C(Predicted) | [density ]
1.52±0.1 g/cm3(Predicted) | [storage temp. ]
Store at -20°C | [solubility ]
DMF:20.0(Max Conc. mg/mL);47.23(Max Conc. mM)
DMSO:10.0(Max Conc. mg/mL);23.62(Max Conc. mM)
Ethanol:10.0(Max Conc. mg/mL);23.62(Max Conc. mM)
Ethanol:PBS (pH 7.2) (1:3):0.5(Max Conc. mg/mL);1.18(Max Conc. mM) | [form ]
Pale yellow liquid. | [pka]
9.61±0.20(Predicted) | [color ]
Colorless to light yellow |
| Hazard Information | Back Directory | [Description]
APF is an aromatic amino-fluorescein derivative and fluorescent probe for highly reactive radicals. It has low intrinsic fluorescence, however, upon oxidation by hydroxyl radical, hypochlorite ion, and certain peroxidase intermediates, it is converted to the highly fluorescent molecule fluorescein, facilitating the detection of highly reactive biological radicals. APF is not oxidized by nitric oxide (NO), hydrogen peroxide (H2O2), or other oxidants. Fluorescein displays excitation/emission maxima of 490/515 nm, respectively. | [Uses]
APF is a fluorescence probe that can selectively, and dose dependently detect certain species among ROS and that are highly resistant to autoxidation. APF can be used in enzymatic and cellular systems[1]. | [storage]
Store at -20°C | [References]
[1] Setsukinai K, et al. Development of novel fluorescence probes that can reliably detect reactive oxygen species and distinguish specific species. J Biol Chem. 2003;278(5):3170-3175. DOI:10.1074/jbc.M209264200 |
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