| Identification | Back Directory | [Name]
RESORUFIN SODIUM SALT | [CAS]
34994-50-8 | [Synonyms]
RESORUFIN
resorufin sodium
RESORUFIN SODIUM SALT
NSC 12097 sodium salt
RESORUFIN, FOR MICROSCOPY
Resorufin Sodium Salt ~90%
7-Sodiooxy-3H-phenoxazin-3-one
Sodium 3-oxo-3H-phenoxazin-7-olate
Resorufin, sodium salt *CAS 34994-50-8*
RESORUFIN SODIUM SALT (DYE CONTENT 75%)
7-HYDROXY-3H-PHENOXAZIN-3-ONE SODIUM SALT
3H-Phenoxazin-3-one, 7-hydroxy-, sodium salt
RESORUFIN, SODIUM SALT *FLUORESCENCE REFERENCE STANDARD*
Resorufin Sodium Salt(Fluorescene Reference Standard)
Resorufin, Sodium Salt *Fluorescence Reference Standa | [EINECS(EC#)]
252-310-2 | [Molecular Formula]
C12H6NNaO3 | [MDL Number]
MFCD00005037 | [MOL File]
34994-50-8.mol | [Molecular Weight]
235.17 |
| Chemical Properties | Back Directory | [Melting point ]
>300 °C(lit.)
| [storage temp. ]
room temp | [solubility ]
Methanol (Slightly), Water (Slightly) | [form ]
Solid | [color ]
Dark Purple to Black | [BRN ]
174850 |
| Hazard Information | Back Directory | [Chemical Properties]
Dark Purple Solid | [Uses]
Resorufin is a pink fluorescent dye that is not fluorescent until activated by UV light. Resorufin is an anionic tricyclic dye. | [General Description]
Resorufin is a fluorescent indicator. | [Purification Methods]
The free acid is purified by dissolving it in conc H2SO4 which on dilution precipitates resorufin and is filtered off, washed well with hot H2O and dried. [Eichler J Prakt Chem 139 113 1934.] Detailed UV and IR studies were reported by Musso & Mattheis Chem Ber 90 1814 1957]. [pKa: Musso & Rathjen Chem Ber 92 751 1959, Beilstein 27 1V 2263.]
The monoacetate [1152-14-3] M 252.2, m 2 2 3o is a fluorogenic substrate used for hydrolytic enzymes, and its fluorescence has Emax 593nm (Eex 500nm, in 0.1M phosphate pH 8.0 with lipase) [Kitson & Kitson Biochem J 322 701 1997]. [Beilstein 27 IV 2263.]
The O7-methylresorufin (7-methoxy-3H-phenoxazin-3-one) [5735-89-3] M 227.2, m > 2 2 0o has fluorescence at Emax 585nm (Eexcit 571 nm in deacylase solution) and is used to differentiate isoenzymes of cytochrome P-450. It is insoluble in H2O and dilute alkali but is soluble in EtOH and CHCl3 to give an orange yellow colour. [Kehrmann Justus Liebigs Ann Chem 372 352 1910, Beilstein 27 II 108.] |
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