| Identification | Back Directory | [Name]
NBD Sphingosine | [CAS]
1449370-25-5 | [Synonyms]
4-Octadecene-1,3-diol, 2-amino-18-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-, (2S,3R,4E)- | [Molecular Formula]
C24H39N5O5 | [MOL File]
1449370-25-5.mol | [Molecular Weight]
477.6 |
| Chemical Properties | Back Directory | [Boiling point ]
674.7±65.0 °C(Predicted) | [density ]
1.200±0.06 g/cm3(Predicted) | [solubility ]
Ethanol: 1 mg/ml | [form ]
Liquid | [pka]
11.80±0.45(Predicted) | [color ]
Colorless to light yellow |
| Hazard Information | Back Directory | [Description]
NBD sphingosine is a biologically active derivative of sphingosine that is tagged with a fluorescent nitrobenzoxadiazole (NBD) group. It has been used to study the cellular localization and metabolism of sphingosine in HeLa cells. NBD sphingosine inhibits proliferation of HCT116, PC3, HEK293T, and HFE-145 cells (IC50s = 25.6, 40.5, 10.4, and 12.5 μM, respectively). | [Uses]
NBD Sphingosine (NBD-Sph), a fluorochrome, is a fluorescence-labeled sphingosine. NBD Sphingosine can be uesd for fluorescence assay for sphingosine kinases[1][2]. | [References]
[1] Bandhuvula P, Fyrst H, Saba JD. A rapid fluorescence assay for sphingosine-1-phosphate lyase enzyme activity. J Lipid Res. 2007 Dec;48(12):2769-78. DOI:10.1194/jlr.D700010-JLR200
[2] Lima S, Milstien S, Spiegel S. A real-time high-throughput fluorescence assay for sphingosine kinases. J Lipid Res. 2014 Jul;55(7):1525-30. DOI:10.1194/jlr.D048132 |
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