| Identification | Back Directory | [Name]
YS 49 | [CAS]
132836-42-1 | [Synonyms]
YS 49
CS-884
YS 49;YS49
1,2,3,4-Tetrahydro-1-(1-naphthalenylmethyl)-6,7-isoquinolinediol hydrobromide
1,2,3,4-Tetrahydro-1-(1-naphthalenylmethyl)-6,7-Isoquinolinediol hydrobromide monohydrate | [Molecular Formula]
C20H20BrNO2 | [MDL Number]
MFCD16875442 | [MOL File]
132836-42-1.mol | [Molecular Weight]
386.282 |
| Hazard Information | Back Directory | [Biological Activity]
YS-49 is an activator of PI3K/Akt, a downstream target of RhoA, and reduces RhoA/PTEN activation in 3-methylcholine-treated cells. YS-49 inhibits angiotensin II (Ang II)-stimulated VSMC proliferation by inducing heme oxygenase (HO-1). YS-49 is an isoquinoline compound alkaloid with strong positive inotropic effects due to its ability to activate cardiac β-adrenoceptors. | [in vitro]
YS-49 (1-100 μM; 18 hours; RAVSMC and RAW 264.7 cells) concentration-dependently inhibits the accumulation of nitrite in both RAVSMC and RAW 264.7 exposed to lipopolysaccharide (LPS) plus INF-γ, with IC 50 values of 22 μM and 30 μM, respectively.
YS-49 (10-100 μM; 18 hours; RAVSMC and RAW 264.7 cells) suppresses iNOS gene expression induced by LPS and/or cytokines in RAVSMC and RAW 264.7 cells at the transcriptional level. Cell Viability Assay | Cell Line: | RAVSMC and RAW 264.7 cells | | Concentration: | 10 μM, 30 μM and 100 μM (RAVSMC cells); 1 μM, 10 μM and 100 μM (RAW 264.7 cells) | | Incubation Time: | 18 hours | | Result: | Inhibited the accumulation of nitrite in both RAVSMC and RAW 264.7 exposed to LPS+INF-γ, with IC 50 values of 22 and 30 μM, respectively . | Western Blot Analysis | Cell Line: | RAVSMC and RAW 264.7 cells | | Concentration: | 10 μΜ, 30 μΜ and 100 μΜ | | Incubation Time: | 18 hours | | Result: | Concentration -dependently inhibited the expression of iNOS protein induced by LPS plus IFN-γ. | | [in vivo]
YS-49 (5 mg/kg; intraperitoneal injection; 8 hours; male Sprague Dawley rats) treatment significantly reduces serum NOx levels in LPS-treated rats, the NOx levels reduce from 86 μM to 34 μM. < /p> < tr> | Animal Model: | Male Sprague Dawley rats (250-300 g) | | Dosage: | 5 mg/kg | | Administration: | Intraperitoneal injection; 8 hours | | Result: | Serum NOx levels were significantly reduced. | |
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