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ChemicalBook--->CAS DataBase List--->129179-83-5

129179-83-5

129179-83-5 Structure

129179-83-5 Structure
IdentificationBack Directory
[Name]

BIOTIN HPDP
[CAS]

129179-83-5
[Synonyms]

Biotin Impurity 31
Pyridyldithiol-biotin
Biotin HPDP,BiotinHPDP
Pyridyldisulfide-biotin
Macamide Impurity 11(Biotin HPDP)
N-[6-(Biotinamido)hexyl]-3'-(2'-pyridyldithio)propionamide
N-[6-(BiotinaMido)hexyl]-3`-(2`-pyridyldithio)propionaMide(Biotin-HPDP)
(3aS,4S,6aR)-Hexahydro-2-oxo-N-[6-[[1-oxo-3-(2-pyridinyldithio)propyl]amino]hexyl]-1H-thieno[3,4-d]imidazole-4-pentanamide
5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(6-(3-(pyridin-2-yldisulfanyl)propanamido)hexyl)pentanamide
1H-Thieno[3,4-d]imidazole-4-pentanamide, hexahydro-2-oxo-N-[6-[[1-oxo-3-(2-pyridinyldithio)propyl]amino]hexyl]-, (3aS,4S,6aR)-
[Molecular Formula]

C24H37N5O3S3
[MDL Number]

MFCD00078541
[MOL File]

129179-83-5.mol
[Molecular Weight]

539.78
Chemical PropertiesBack Directory
[Melting point ]

160-163°C
[Boiling point ]

876.0±65.0 °C(Predicted)
[density ]

1.28
[storage temp. ]

-20°C Freezer
[solubility ]

insoluble in H2O; ≥101.4 mg/mL in DMSO; ≥8.29 mg/mL in EtOH with ultrasonic
[form ]

powder to crystal
[pka]

13.90±0.40(Predicted)
[color ]

White to Light yellow
Safety DataBack Directory
[HS Code ]

2934.99.4400
Hazard InformationBack Directory
[Description]

Biotin-HPDP is a sulfhydryl-reactive biotinylation reagent that forms a reversible disulfide linkage. It is used to label protein cysteines and other substrates that contain sulfhydryl groups. Biotin-HPDP is also used in the biotin switch technique to tag S-nitrosylated (SNO) proteins, following reduction of SNO groups to thiols. Compounds that are tagged with biotin interact avidly with streptavidin-coupled beads, fluorophores, enzymes, etc. The interaction of biotin-HPDP with substrates containing sulfhydryl groups is easily performed at pH 6.5 to 7.5 in buffers such as PBS. The disulfide linkage that is formed between avidin and substrate can later by cleaved by a reducing agent, like dithiothreitol.
[Chemical Properties]

Pale Yellow Solid
[Uses]

This compounds reacts specifically with thiol groups.
Spectrum DetailBack Directory
[Spectrum Detail]

BIOTIN HPDP(129179-83-5)1HNMR
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